摘要
目的引入样品前处理方法,建立一个用甲醛吸收液-盐酸副玫瑰苯胺比色法测定葡萄酒中游离二氧化硫的方法。方法在一定温度和酸性条件下,通入空气,葡萄酒样品中的游离二氧化硫随空气进入吸收管被甲醛吸收液吸收后,生成稳定的羧甲基磺酸,在加入氢氧化钠后释放出二氧化硫,与盐酸副玫瑰苯胺反应生成红色化合物,比色定量。结果用本文的样品前处理方法对葡萄酒样品进行处理后,溶液中存在的颜色、还原性物质等均被除去,对测定结果无干扰。同时由于样品被浓缩,用本比色法测定游离二氧化硫含量还可以降低检出限。本法回收率为92.5%~96.8%,相对标准偏差为0.5%~1.81%。结论本文建立的样品前处理方法和比色法测定葡萄酒中游离二氧化硫含量,其测定结果准确度高、灵敏度高、精密度好、可靠且实用性强。
Objective To establish a colorimetry method for free sulfur dioxide determination in wine with reagents of formaldehyde absorption solution - pararosaniline hydrochloride and a new sample pretreatment procedure. Methods Free sulfur dioxide was generated along with atmosphere through air introduction. Then it entered an absorption tube,where it reacted with formaldehyde and a stable product of hydroxyl methanesulfonic acid was produced at the specified temperature and acidity. Sulfur dioxide was released with the addition of sodium hydroxide. A new compound with red color was obtained with the reaction between the released sulfur dioxide and pararosaniline hydrochloride,and then it was determined by colorimetry. Results Both the color and the reducing substances were eliminated with the pretreatment procedure proposed in this paper and the analytical results were no more interfered. Moreover,the detection limit for free sulfur dioxide was further decreased as the sample was concentrated in the pretreatment procedure. The recoveries ranged from 92.5% to 96.8% with the relative standard deviations( RSDs) of 0.5% - 1.81%. Conclusion The established sample pretreatment and colorimetry procedure in determination of free sulfur dioxide in wine was of high accuracy,high sensitivity and good precision as well as excellent practicability.
作者
邱宏
肖义夫
钟汉怀
QIU Hong;XIAO Yi -fu;ZHONG Han -huai(Chongqing Center for Disease Control and Prevention,Chongqing 400042,China)
出处
《中国卫生检验杂志》
CAS
2018年第24期2968-2970,共3页
Chinese Journal of Health Laboratory Technology
关键词
葡萄酒
游离二氧化硫
比色法
研究
Wine
Free sulfur dioxide
Colorimetric method
Determination
