摘要
目的探讨乳腺癌根治术患者miRNA-23a表达水平与术后复发转移的关系。结果选取2013年3月至2016年3月本院收治的乳腺癌根治术患者160例,依据2年内复发转移情况分为复发组(n=50例)和未发组(n=110例),采用实时荧光定量聚合酶联反应法(RT-PCR)检测miRNA-23a表达水平,分析miRNA-23a表达水平与术后复发转移的关系。结果单因素分析结果显示,复发组和未发组肿瘤类型、直径、TNM分期、淋巴结转移、雌激素受体(ER)和孕激素受体(PR)、miRNA-23a表达水平比较,差异有统计学意义(P<0.05);Logistic回归性分析显示,在校正肿瘤类型、直径、TNM分期、淋巴结转移、ER和PR等因素后,miRNA-23a表达水平是患者术后复发转移的独立影响因素(P<0.05);ROC曲线显示,miRNA-23a表达水平以<1.0评估患者术后复发转移的敏感度、特异度、准确度、曲线下面积为92.00%、96.36%、95.00%、0.882,一致性良好(P<0.05)。结论乳腺癌根治术患者miRNA-23a表达水平与术后复发转移的关系密切,其低表达水平可能提示患者术后复发转移,检测其表达水平可作为评估患者术后复发转移的重要指标。
Objective To discuss the relationship between miRNA-23a expression level and postoperative recurrence metastasis in patients undergoing breast cancer radical mastectomy.Methods 160patients undergoing breast cancer radical mastectomy were selected from March 2013 to March 2016in our Hospital,according to the recurrence metasta- sis within 2 years,they were divided into the recurrence group (n=50)and the non recurrence group (n=110),the miRNA-23a expression level were detected by real time fluorescence quantitative polymerase chain reaction (RT-PCR), and the relationship between the miRNA-23a expression level and the postoperative recurrence metastasis was ana- lyzed.Results The single factor analysis result showed that,the comparison of the tumor type,diameter,TNM stag- ing,lymph node metastasis,estrogen receptor (ER)and progesterone receptor (PR)and miRNA-23a expression level in the recurrence group and the non recurrence group,the difference was statistically significant (P<0.05).The Logistic regression analysis showed that,the tumor type,diameter,TNM staging,lymph node metastasis,ER and PR were cor- rected,the miRNA-23a expression level was an independent factor for the postoperative recurrence metastasis of pa- tients (P<0.05).The ROC curve showed that,the sensitivity,specificity,accuracy,area under the curve of miRNA-23a expression level <1.0for evaluating the postoperative recurrence metastasis of patients were 92.00%,96.36%,95. 00%,0.882,it had good consistency (P<0.05).Conclusion The miRNA-23a expression level in patients undergoing breast cancer radical mastectomy is closely related to postoperative recurrence metastasis,its low expression level may indicate the postoperative recurrence and metastasis of patients,the detection of its expression level can be used as the important index for evaluating the postoperative recurrence metastasis of patients.
作者
周永安
赵德庆
ZHOU Yong-an;ZHAO De-qing(Department of Breast Cancer Surgery,Huangshi Central Hospital ,A f ficiated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435001,China;Department of Thoracic Oncology ,Huangshi Central Hospital ,A f ficiated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435001,China)
出处
《中国实验诊断学》
2018年第12期2049-2053,共5页
Chinese Journal of Laboratory Diagnosis
