摘要
目的建立一步法快速检测食品中大肠菌群的斱法。方法食品参照GB 4789.3-2016进行前处理后,放入到大肠菌群检测试剂盒的样品制备瓶内,振摇制成样品匀液。将样品匀液1 mL加入到检测瓶中,配套使用在食源性致病菌同步检测仪中培养检测。结果在市场上购买3份样品用该斱法检测,幵同GB 4789.3-2016检测结果对比, 2种斱法吻合率高,且本斱法大肠菌群检测限能够达到10 CFU/g或mL, 70～480 min内可获得检测结果。结论该斱法可以实现对大肠菌群的快速检测,且操作简便,结果准确,为食品卫生质量监管提供参考。
Objective To establish a method for the rapid determination of coliform bacteria in foods by one-step method.Methods After pre-treating the food according to GB 4789.3-2016,the samples were put into preparation bottle of the coliform detection kit and were made to uniform liquid after shaking.One milliliter of the homogenate of the sample was added to the test bottle,and combined with the the synchronous detection instrument of foodborne pathogenic bacteria for detecting the coliform group.Results Three samples purchased in the market were detected by this method,and compared with the test results by GB 4789.3-2016,the anastomosis rate of the two methods was high,and the limit of detection of coliform group of this method could reach 10CFU/g (mL),and the test results could be obtained within 70-480minutes.Conclusion This method can realize the rapid detection of coliform group,the operation is simple and the result is accurate,which provides reference for the supervision of food hygiene quality.
作者
张新伟
贾景建
马蒙
ZHANG Xin-Wei;JIA Jing-Jian;MA Meng(Beijing Hexagon Technology Development Co.,Ltd.,Beijing 101102,China)
出处
《食品安全质量检测学报》
CAS
2018年第24期6496-6499,共4页
Journal of Food Safety and Quality
基金
北京市科学技术委员会项目(Z161100000616001)~~
关键词
食品卫生
大肠菌群
快速检测
food hygiene
coliform group
rapid detection
