摘要
为探究葛根素(Pur)在镉(Cd)致大鼠大脑皮质氧化损伤中的保护作用,40只雄性SD大鼠随机分为4组,分别为对照组、镉组、葛根素组、镉与葛根素共处理组。试验期间,所有大鼠均自由饮用纯净水,每天按200 mg/kg剂量的葛根素对葛根素组、镉与葛根素共处理组大鼠进行灌胃,同时以相同剂量的纯净水将对照组、镉组大鼠进行灌胃,连续处理5周,镉组、镉与葛根素共处理组大鼠在处理4周后连续一周每天用醋酸镉按2.5 mg/kg剂量进行腹腔注射。5周后,分离大鼠大脑皮质,测定大脑皮质内总抗氧化能力(T-AOC),超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的含量。结果表明,与对照组相比,镉组大鼠大脑皮质T-AOC,SOD和GSH-Px活性显著降低(P<0.05),MDA含量显著升高(P<0.05);与镉组相比,镉与葛根素共处理组T-AOC,SOD和GSH-Px活性显著升高(P<0.05),MDA含量显著降低(P<0.05)。说明葛根素在镉致大鼠大脑皮质氧化损伤中具有一定的保护作用。
In order to evaluate the oxidative damage induced by cadmium exposure in cerebral cortex of SD rats and protective effects of puerarin,forty male SD rats(70±5) g were randomly divided into four groups:control group,Cd-treated group,puerarin group,Cd and puerarin co-treated group.All rats were free acess to purified water during the experiment.When puerarin(200 mg/kg) was given to rats by gavage in the puerarin group and the co-treated group,the same dose of purified water was given to rats by gavage concurrently in the control group and the Cd-treated group over 5 weeks.The Cd Ac2(2.5 mg/kg) was given to rats by intraperitoneal injection daily over 1 week at the fifth week in the Cd group and the co-treated group.Five weeks later,the cerebral cortex was isolated.The T-AOC,the activities of SOD and GSH-Px and the contents of MDA in cerebral cortex were measured.In result,compared with the control group,the T-AOC,the activities of SOD and GSH-Px in cadmium-exposed groups all decreased significantly(P<0.05),and the contents of MDA in cerebral cortex increased significantly(P<0.05).Compared with the cadmium group,the T-AOC,the activities of SOD and GSH-Px in co-treated groups all increased significantly(P<0.05),and the contents of MDA decreased significantly(P<0.05).The results indicated that puerarin can protect the oxidative damage caused by cadmium in cerebral cortex of rats.
作者
闻双全
赵世文
陈洁
朱桥平
邹辉
顾建红
刘学忠
卞建春
刘宗平
袁燕
WEN Shuangquan;ZHAO Shiwen;CHEN Jie;ZHU Qiaoping;ZOU Hui;GU Jianhong;LIU Xuezhong;BIAN Jianchun;LIU Zongping;YUAN Yan(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009, China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China)
出处
《畜牧与兽医》
北大核心
2018年第12期104-107,共4页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金资助项目(31772808)
中国博士后科学基金资助项目(2015M581874)
江苏省博士后科研资助计划项目(1501072A)
国家重点研发计划支持项目(2016YFD0501208)
江苏高校优势学科建设工程资助项目(PAPD)
关键词
葛根素
镉
SD大鼠
大脑皮质
氧化损伤
puerarin
cadmium
SD rat
cerebral cortex
oxidative damage
