摘要
目的建立HPLC测定脑心清流膏及脑心清片中槲皮苷含量的方法。方法色谱柱为Agilent Eclipse Plus C_(18)柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.1%磷酸水溶液(B)为流动相进行梯度洗脱,0～40 min,7%→25%A,40～60 min,25%→50%A;流速为1.0mL·min^(-1);检测波长为360 nm;柱温为30℃。结果槲皮苷浓度在19.4～775.7μg·mL^(-1)内与峰面积线性关系良好(r=0.9999),脑心清流膏和脑心清片平均加样回收率(n=6)分别为100.07%和100.16%,RSD分别为1.88%和1.45%。结论该方法操作简便、结果准确、重现性好,可作为脑心清流膏及其片剂中槲皮苷的含量测定方法。
OBJECTIVE To establish a method of determine the content of quercitrin in Naoxinqing crude drug and tablets by HPLC.METHODS The samples were separated on an Agilent Eclipse Plus C 18 column (250 mm×4.6 mm,5 μm),by a gradient elution using the mobile phase of acetonitrile (A)-0.1% phosphoric acid (B) (0~40 min,7%→25%A; 40~60 min,25%→ 50%A) at a flow rate of 1.0 mL·min^-1.The detection wavelength was set on 360 nm and the column temperature was set at 30 ℃.RESULTS A good linearity was observed for quercitrin in the range of 19.4775.7 μg·mL^-1 (r=0.999 9).The average recoveries(n=6) of Naoxinqing crude drug and tablets were 100.07% and 100.16% with the RSD of 1.88% and 1.45%,respectively.CONCLUSION The method is simple,accurate,reproducible,and applicable for the content determination of quercitrin in Naoxinqing crude drug and tablets.
作者
苏诗韵
郭海彪
李楚源
王德勤
黄雁
匡艳辉
蔡越冬
SU Shiyun;GUO Haibiao;LI Chuyuan;WANG Deqin;HUANG Yan;KUANG Yanhui;CAI Yuedong(Guangzhou Baiyun Mountain and Hutchison Whampoa Ltd.,Gaungzhou 510515,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2018年第12期1874-1877,共4页
Chinese Journal of Modern Applied Pharmacy
基金
广东省科技计划项目(2011A080502005)
