摘要
【目的】了解粉红粘帚霉对楚雄腮扁叶蜂幼虫的侵染过程,为粉红粘帚霉对楚雄腮扁叶蜂的致病机理研究提供参考。【方法】利用扫描电镜和透射电镜技术对粉红粘帚霉SWFUYHL 02-03孢子侵染楚雄腮扁叶蜂幼虫体表过程及其超微结构的变化进行观察研究。【结果】扫描电镜结果显示,接种6 h后,粉红粘帚霉SWFUYHL 02-03分生孢子成功附着于幼虫体壁的气门、节间膜等部位;接种14 h后,粉红粘帚霉SWFUYHL 02-03孢子萌发出芽管,并侵入体壁;接种26 h后,孢子和菌丝穿透楚雄腮扁叶蜂幼虫体壁,且菌丝迅速生长;接种72 h后,菌丝穿出虫体表面,并长出产孢结构。透射电镜观察发现粉红粘帚霉SWFUYHL 02-03菌丝在穿透幼虫体壁过程中,会伴随酶的参与和组织溶解现象;接种6 h后,粉红粘帚霉SWFUYHL 02-03分生孢子附着于幼虫体壁的两个节间褶间的部位;接种14 h后,分生孢子萌发并进入表皮细胞层分泌物中;接种26 h后,菌丝侵入表皮并扩展蔓延,且在菌丝周围出现电子密度很低的光晕;接种38 h后,皮细胞层细胞排列松弛变成空泡;接种48 h后,菌丝充满虫体,表皮层出现大量菌丝。【结论】粉红粘帚霉SWFUYHL 02-03孢子及菌丝能寄生楚雄腮扁叶蜂幼虫,并可致其死亡。观察结果准确反映了粉红粘帚霉SWFUYHL 02-03对楚雄腮扁叶蜂幼虫的侵染过程,并证实了侵染过程中酶与代谢产物的存在。
[Objective]This paper aims to study the process of Clonostachys rosea infecting Cephalcia chuxiongica larvae, and provides a reference for the research on pathogenicity mechanism of Ce. chuxiongica.[Method] We observed the ultrastructural changes of Ce. chuxiongica body wall infected by Cl. rosea SWFUYHL 02-03 using the scanning electronic microscopy (SEM) and transmission electron microscopy (TEM).[Result] Based on the SEM observation, the conidias adhered onto spiracles and internode membranes of body wall after 6 hours inoculation. The germ tubes of this fungi penetrated into the cuticle of Ce. chuxiongica larvae after 14 hours. The conidia and hypha penetrated into the cuticle of Ce. chuxiongica larvae and the hypha began to multiply rapidly after 26 hours, and then the hypha penetrated out from the cuticle of Ce. chuxiongica larvae and generated sporogenous structure when it was inoculated at 72 hours. Meanwhile, inferred from the TEM observation, the participation of enzymes and histolysis could be found with the penetration process of hypha. The spores adhered onto the body wall of the Ce. chuxiongica larvae when it was inoculated at 6 hours and then the conidia germinated and penetrated into the secretion of cuticle after inoculation for 14 hours. The hypha penetrated and spreaded into the cuticle, and halos with low electron density could be observed around the penetrated hypha after 26 hours. The epidermis cell arranged loose and became vacuole after inoculation for 38 hours and the larvae's body was full of hypha, and a great deal of hypha were on the cuticle of Ce. chuxiongica larvae after inoculation for 48 hours.[Conclusion] The spores and hypha of Cl. rosea SWFUYHL 02-03 could parasitize and kill the Ce. chuxiongica larvae. The SEM and TEM observations accurately reflected the infection process of Cl. rosea SWFUYHL 02-03 on the larvae of Ce. chuxiongica,and proved the existence of enzymes and virulent metabolites.
作者
芦俊佳
徐荣
李永和
Lu Junjia;Xu Rong;Li Yonghe(College of Biodiversity Conservation and Utilization,Southwest Forestry University,Kunming 650224,Yunnan,China)
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2018年第12期68-75,共8页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(31360156)
西南林业大学云南省省级重点学科(林学)建设项目
国家级省级大学省创新创业训练计划项目(516027)
关键词
粉红粘帚霉SWFUYHL
02—03
楚雄腮扁叶蜂
侵染过程
扫描电镜
透射电镜
Clonostachys rosea SWFUYHL 02-03
Cephalcia chuxiongica
infection process
scanning electron microscopy (SEM)
transmission electron microscopy (TEM)
