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The difference of morphological characteristics and population structure in PAO and DPAO granular sludges 被引量:6

The difference of morphological characteristics and population structure in PAO and DPAO granular sludges
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摘要 We examined how long-term operation of anaerobic–oxic and anaerobic–anoxic sequencing batch reactors(SBRs) affects the enhanced biological phosphorus removal(EBPR)performance and sludge characteristics. The microbial characteristics of phosphorus accumulating organism(PAO) and denitrifying PAO(DPAO) sludge were also analyzed through a quantitative analysis of microbial community structure. Compared with the initial stage of operation characterized by unstable EBPR, both PAO and DPAO SBR produced a stable EBPR performance after about 100-day operation. From day 200 days(DPAO SBR)and 250 days(PAO SBR) onward, sludge granulation was observed, and the average granule size of DPAO SBR was approximately 5 times larger than that of PAO SBR. The DPAO granular sludge contained mainly rod-type microbes, whereas the PAO granular sludge contained coccus-type microbes. Fluorescence in situ hybridization analysis revealed that a high ratio of Accumulibacter clade I was found only in DPAO SBR, revealing the important role of this organism in the denitrifying EBPR system. A pyrosequencing analysis showed that Accumulibacter phosphatis was present in PAO sludge at a high proportion of 6%,whereas it rarely observed in DPAO sludge. Dechloromonas was observed in both PAO sludge(3.3%) and DPAO sludge(3.2%), confirming that this organism can use both O_2 and NO_3^- as electron acceptors. Further, Thauera spp. was identified to have a new possibility as denitrifier capable of phosphorous uptake under anoxic condition. We examined how long-term operation of anaerobic–oxic and anaerobic–anoxic sequencing batch reactors(SBRs) affects the enhanced biological phosphorus removal(EBPR)performance and sludge characteristics. The microbial characteristics of phosphorus accumulating organism(PAO) and denitrifying PAO(DPAO) sludge were also analyzed through a quantitative analysis of microbial community structure. Compared with the initial stage of operation characterized by unstable EBPR, both PAO and DPAO SBR produced a stable EBPR performance after about 100-day operation. From day 200 days(DPAO SBR)and 250 days(PAO SBR) onward, sludge granulation was observed, and the average granule size of DPAO SBR was approximately 5 times larger than that of PAO SBR. The DPAO granular sludge contained mainly rod-type microbes, whereas the PAO granular sludge contained coccus-type microbes. Fluorescence in situ hybridization analysis revealed that a high ratio of Accumulibacter clade I was found only in DPAO SBR, revealing the important role of this organism in the denitrifying EBPR system. A pyrosequencing analysis showed that Accumulibacter phosphatis was present in PAO sludge at a high proportion of 6%,whereas it rarely observed in DPAO sludge. Dechloromonas was observed in both PAO sludge(3.3%) and DPAO sludge(3.2%), confirming that this organism can use both O_2 and NO_3^- as electron acceptors. Further, Thauera spp. was identified to have a new possibility as denitrifier capable of phosphorous uptake under anoxic condition.
出处 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2019年第2期388-402,共15页 环境科学学报(英文版)
基金 supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (No.NRF-2015R1D1A1A01061423)
关键词 Accumulibacter phosphatis Dechloromonas GRANULATION PYROSEQUENCING THAUERA Accumulibacter phosphatis Dechloromonas Granulation Pyrosequencing Thauera
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  • 1ZHUGui-bing,PENGYong-zhen,WUShu-yun,WANGShu-ying.Automatic control strategy for step feed anoxic/aerobic biological nitrogen removal process[J].Journal of Environmental Sciences,2005,17(3):457-459. 被引量:4
  • 2张洁,田立江,张雁秋.反硝化聚磷菌群的培养驯化[J].环境污染治理技术与设备,2006,7(5):74-77. 被引量:15
  • 3张小玲,张立卿,袁林江,王磊,王志盈.硝酸盐浓度对反硝化聚磷菌诱导的影响[J].中国给水排水,2006,22(13):105-108. 被引量:16
  • 4Kuba T, Van Loosdrecht M C M, Heijinen J J. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system[J]. Water Res,1996,30(7): 1702-1710.
  • 5Meinhold J,Filipe C D M, Daigger G T,et al. Characterization of the denitrifying fraction of phosphate accumulating organisms in biological phosphate removal[ J]. Water Sci Technol, 1999, 39 (1) : 31-42.
  • 6Lee D S, Jeon C O, Park J M. Biological nitrogen removal with enhanced phosphate uptake in a sequencing batch reactor using single sludge system [J]. Water Res, 2001,35(16): 3968- 3976.
  • 7Kishida N, Kim J, Tsuneda S, et al. Anaerobic/oxic/anoxic granular sludge process as an effective nutrient removal process utilizing denitrifying polyphosphate-accumulating organisms [ J ]. Water Res, 2006,40(12) : 2303-2310.
  • 8Mino T, Van Loosdrecht M C M, Heijnen J J. Microbiology and biochemistry of the enhanced biological phosphate removal process [ J]. Water Res,1998, 32(11 ) : 3193-3207.
  • 9Jian Y, Jian P W. Metabolic flux modeling of detoxification of acetic acid by Ralstonia eutropha at slightly alkaline pH levels [ J ]. Biotechnol Bioeng,2001,73 : 458-464.
  • 10Waehtmeister A, Kuba T, Van Loosdrecht M C M. A sludge characterization for aerobic and denitrifying phosphorus removingsludge [ J ]. Water Res, 1997, 31 (3) : 471-478.

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