摘要
目的分析比较BP180抗体在大疱性类天疱疮(BP)与神经系统疾病(ND)患者血清中的表达差异,探讨BP与ND关系。方法收集2012年3月至2013年9月吉林大学第二医院皮肤科门诊及住院BP患者和神经科住院ND患者的临床资料。采集BP患者、ND患者、BP + ND患者及健康对照(各20例)外周血,ELISA法测定各组BP180抗体数值。应用SPSS17.0软件对数据进行统计学分析。计数资料比较采用卡方检验;各组年龄、BP180滴度比较采用方差分析,两两比较采用Games-Howell检验。结果 健康对照组20例BP180抗体均阴性;BP组20例患者BP180抗体均为阳性,滴度为128.347±54.678;BP + ND组20例患者所有结果均为阳性,滴度为143.482±72.568;ND组20例患者中,7例BP180抗体阳性,包括脑出血4例、脑梗塞3例,其中BP180抗体最高滴度(39.638)出现在1例脑梗塞患者。4组间BP180抗体滴度比较,差异有统计学意义(F=55.624,P<0.05),其中,BP组与BP + ND组间差异无统计学意义(P=0.878),BP组与ND组、BP组与健康对照组、BP + ND组与ND组、BP + ND组与健康对照组、ND组与健康对照组间差异均有统计学意义(P<0.05)。结论ND患者血清BP180抗体增高,可能提示发生BP的可能。
Objective To measure the expression of BP180 antibody in sera of patients with bullous pemphigoid (BP) and/or nervous system diseases (ND) , and to explore the relationship between BP and ND. Methods Clinical data were collected from some inpatients and outpatients with BP in Department of Dermatology, as well as some inpatients with ND in Department of Neurology, the Second Hospital of Jilin University between March 2012 and September 2013. Peripheral blood samples were obtained from 20 BP patients without a medical history of ND (BP group) , 20 patients with ND alone (ND group) , 20 BP patients with a medical history of ND (BP + ND group) , and 20 healthy controls (healthy control group) separately. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the serum level of BP180 antibody in the above groups. Statistical analysis was carried out with SPSS17.0 software by using chi-square test for comparing enumeration data, analysis of variance for comparing the anti-BP180 antibody titer or ages among the above 4 groups, and Games-Howell test for multiple comparisons. Results No anti-BP180 antibody was detected in any of the 20 subjects in the healthy control group. All of the patients in the BP group and BP + ND group were positive for anti-BP180 antibody, and the antibody titers were 128.347±54.678 and 143.482±72.568 respectively. Of the 20 patients in the ND group, 7 were positive for anti-BP180 antibody, including 4 with cerebral hemorrhage and 3 with cerebral thrombosis, and the highest anti-BP180 antibody titer of 39.638 was recorded in 1 patient with cerebral infarction. There was a significant difference in the anti-BP180 antibody titer among the 4 groups (F=55.624, P<0.05) . In addition, there was no significant difference in the anti-BP180 antibody titer between the BP group and BP + ND group (P=0.878) , while the anti-BP180 antibody titers significantly differed between the BP group and ND group, between the BP group and healthy control group, between the BP + ND group and ND group, between the BP + ND group and healthy control group, and between the ND group and healthy control group (P<0.05) . Conclusion BP may occur in ND patients with an increased anti-BP180 antibody titer.
作者
朱磊
李博平
巫毅
Zhu Lei;Li Boping;Wu Yi(Department of Dermatology,Jilin Province People's Hospital,Changchun 130000,China;Department of Dermatology,The Second Hospital of Jilin University,Changehun 130041,China)
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2019年第1期33-35,共3页
Chinese Journal of Dermatology
