摘要
以三叶草(Trifolium repens)为供试植物,利用室内盆栽试验,研究土壤加硒(Se)对不同柴油(diesel)质量分数梯度(0、15、20、25g/kg)胁迫下三叶草光合荧光特性的影响,以期探索出一种提高植物在高质量分数柴油胁迫下植物生长的方法,支持补充植物修复技术.结果表明:不同柴油质量分数梯度对三叶草的根茎鲜重有显著的抑制(P<0.05),三叶草光合荧光特性在柴油的作用下受到明显的抑制,表现为PSII最大光化学效率(Fv/Fm)、PSII实际光化学效率(ФPSⅡ)、光化学淬灭系数(qP)、净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)随着柴油质量分数的增加显著降低(P<0.05),胞间二氧化碳质量分数(Ci)和非光化学淬灭系数(qN)随着柴油质量分数升高逐渐升高.而加硒显著缓解了柴油对植物生长和光合作用的胁迫,表现为根茎鲜重最大分别提高了59.4%、28.9%;光合荧光参数Fv/Fm、ФPSⅡ、qP、Pn、Tr和Gs相对于无硒对照组显著增加,Pn增加了18%～49%;Gs增加了24%～32%;Tr增加了6%～31%;Fv/Fm增加了1.4%～3.2%;qP增加了11.4%～21.5%;ФPSⅡ增加了14.4%～21.0%.而Ci相对于不加硒组呈降低趋势,但并不显著,qP降低了0.8%～2.1%.加硒后三叶草的光合参数和叶绿素荧光参数都显著区别于不加硒对照组,表明三叶草的光合作用受抑制的状态在硒的作用下有显著的改善.因此通过向柴油污染土壤添加低质量分数硒,可以有效缓解柴油对植物光合作用的抑制,促进植物生长.研究结果对植物修复柴油污染土壤有一定的支持补充作用.
A pot-culture experiment is conducted to assess the effects of selenium on Trifolium repens exposed to various levels of diesel (0,15,20,25 g/kg).The results show that the fresh weights of both shoot and root all decrease with the increased diesel.Exposure to diesel led to concentration-dependent decreases in photosynthesis fluorescent parameters (except qN and Ci).qN and Ci showed a reverse trend. Application of Se to soil increased Trifoliumrepenstolerance to diesel and significantly increased the photosynthesis fluorescent parameters (Fv/Fm,ФPSⅡ ,qP,Pn,Tr and Gs),with the increments of Pn ranged from 18% to 49%;Gs ranged from 24% to 32% Tr ranged from 6% to 31%;Fv/Fm ranged from 1.4% to 3.2%;qP ranged from 11.4% to 21.5%;ФPSⅡ ranged from 14.4% to 21.0%.qP showed an significant decreased in Se applied groups and Ci showed insignificant decreased in Se applied groups when compared to non-Se applied groups.These results contribute to the ongoing effort to develop an effective phytoremediation system for soils contaminated by diesel.
作者
席颖
刘慧刚
袁喜
张栋栋
任东
黄应平
Xi Ying;Liu Hueigang;Yuan Xi;Zhang Dongdong;Ren Dong;Huang Yingping(College of Hydraulic & Environmental Engineering,China Three Gorges Univ.,Yichang 443002, China;Hubei Provincial Farmland Environment Monitoring Engineering Technology Center,China Three Gorges Univ.,Yichang 443002,China;Engineering Research Center of Eco-environment in Three Gorges Reservoir Region of Ministry of Education,China Three Gorges Univ.,Yichang 443002,China)
出处
《三峡大学学报（自然科学版）》
CAS
北大核心
2019年第1期103-107,共5页
Journal of China Three Gorges University：Natural Sciences
基金
国家重点研发计划(2016YFD0800902)
湖北省创新群体滚动项目(2015CFA021)
湖北省农田环境监测工程技术中心开放基金(201605)
三峡大学学位论文培优基金(2018BSPY001)
关键词
柴油
硒
三叶草
光合
荧光
植物修复
diesel
selenium
Trifolium repens
photosynthesis
fluorescent
phytoremediation
