摘要
目的:制备神经生长因子(NGF)温度敏感型原位凝胶,以大鼠坐骨神经损伤为模型,探讨NGF温敏凝胶对大鼠坐骨神经损伤的修复作用。方法:制备NGF温敏凝胶,利用星点设计-效应面法优化其处方。将50只大鼠随机分为正常组,模型组,NGF注射剂组(10 mg·L^-1),NGF低剂量温敏凝胶组(10 mg·L^-1)和NGF高剂量温敏凝胶组(20 mg·L^-1),建立大鼠坐骨神经损伤模型;以大鼠行为学,坐骨神经功能指数(SFI),撤回反射时间的测定实验,腓肠肌湿重比的测定以及组织形态学变化为指标,综合考察NGF温敏凝胶对损伤坐骨神经的影响。结果:处方优化后NGF温敏凝胶的胶凝温度35. 2℃,符合注射用标准。术后48周,各时间点测得NGF高剂量温敏凝胶组大鼠的SFI和腓肠肌湿重比都显著高于模型组和NGF注射剂组,但其撤回反射时间显著低于模型组和NGF注射剂组,且作用呈剂量依赖性。由苏木精-伊红(HE)染色试验可知,NGF高剂量温敏凝胶组的大鼠坐骨神经损伤区再生神经纤维排列较模型组整齐、密集,而且连续性也较好。结论:NGF温敏凝胶能促进大鼠坐骨神经损伤的修复。
Objective: To prepare nerve growth factor( NGF) temperature sensitive in situ gel and investigate its therapeutic effect on sciatic nerve injury of rats. Method: NGF thermosensitive gel was prepared and its prescription was optimized by central composite design-response surface methodology. Fifty rats were randomly divided into the normal group,model group,NGF injection group( 10 mg·L^-1),NGF low-dose( 10 mg·L^-1)and high-dose( 20 mg·L^-1) thermosensitive gel groups,and sciatic nerve injury model of rats was established.The effect of NGF thermosensitive gel on the injury of sciatic nerve were comprehensively examined by taking rat behavior,sciatic nerve function index( SFI), time of withdrawal reflex, wet weight ratio of gastrocnemius muscle,and histomorphological changes as indicators. Result: The gelation temperature of NGF thermosensitive gel was 35. 2 ℃ after the formulation being optimized,which was in line with the standard for injection. Foureight weeks after operation, the SFI and wet weight ratio of gastrocnemius muscle in rats of NGF high-dose thermosensitive gel group were significantly higher than those in the model group and NGF injection group,but its time of withdrawal reflex was significantly lower than those in the model group and NGF injection group,and the effect was in a dose-dependent manner. Arrangement of regenerated nerve fibers in sciatic nerve injury area of rats from NGF high-dose thermosensitive gel group was more tidy,dense and continuous than that of the model group.Conclusion: NGF thermosensitive gel can promote repair of sciatic nerve injury in rats.
作者
江敏
宋旺弟
陈文
郭亚可
JIANG Min;SONG Wang-di;CHEN Wen;GUO Ya-ke(School of Pharmacy,Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education,Shihezi University,Shihezi 832000,China;The First Affiliated Hospital of the Medical College,Shihezi University,Shihezi 832008,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第3期27-33,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81160395)
关键词
神经生长因子
温度敏感型原位凝胶
星点设计-效应面法
坐骨神经损伤
修复作用
坐骨神经功能指数
撤回反射时间
nerve growth factor
thermosensitive in situ gel
central composite design-response surface methodology
sciatic nerve injury
restorative effect
sciatic nerve function index
time of withdrawal reflex
