摘要
研究新疆冬小麦萌发期对旱胁迫的响应,并进行抗旱性综合评价,为小麦萌发期抗旱品种的评价和筛选提供理论依据。本研究采用20%PEG-6000胁迫剂并利用位于5BS染色体上的功能标记TaNRX-a1和TaNRX-b1分别进行萌发期抗旱鉴定和分子标记检测,从而对83份新疆冬小麦品种(系)进行萌发期抗旱性综合评价。测定小麦发芽率、发芽势、发芽指数、根数、最长根长、苗高和胚芽鞘长度7个指标,结果表明,旱胁迫均值较对照处理分别下降30.86%、23.91%、31.37%、0.43%、31.07%、50.27%和6.61%。在83份新疆冬小麦品种(系)中TaNRX-a和TaNRX-b变异类型频率分别占83.1%和16.9%。具有种子萌发期高抗旱能力等位变异TaNRX-a为优势等位变异。在种子萌发期高抗旱能力的优势等位变异TaNRX-a变异类型的材料中地方品种、引进品种和自育品种的分布频率依次为93.8%、82.1%和79.5%。通过加权隶属函数法及分子标记检测相结合表明,抗旱性最强且具有TaNRX-a变异类型的品种(系)有昌冬5号、旱洋麦、卡衣木红、纳瓦提然和高加索。相关性分析表明,胚芽鞘长、最长根长分别与萌发期综合评价值D值的相关性较大分别为0.557、0.516,可作为小麦抗旱性快速鉴定指标。本研究将表型和分子标记相结合,能更有效地评价小麦萌发期抗旱能力。
The comprehensive evaluation of drought resistance at the germination stage was conducted in winter wheat cultivars from Xinjiang,which provided the theoretical basis for the evaluation and selection of drought resistant wheat varieties during the germination period.In this study,two methods that possessed 20%PEG-6000 treatment and marker-assisted selection(MAS)with the functional markers TaNRX-a1 and TaNRX-b1 located on the 5BS chromosome,were used for adjusting drought resistance in 83 Xinjiang winter wheat cultivars at the germination stage.Seven indexes including germination rate,germinating energy,germination index,root number,root length,seedling height and coleoptile length were measured,and the results suggested that the seven indexes average of drought resistance were decreased by 30.86%,23.91%,31.37%,0.43%,31.07%,50.27%and 6.61%,respectively.The allele frequency of TaNRX-a and TaNRX-b in 83 winter wheat cultivars accounted for 83.1% and 16.9%,respectively.The TaNRX-a allele with high drought resistance during seed germination is predominant,while this allele was observed 93.8%,82.1% and 79.5% of local varieties,introduced varieties and improved varieties,respectively.By applying the weighted membership function method together with MAS for TaNRX-a,the cultivars Changdong 5,Hanyangmai,Kayimuhong,Nawatiran,and Gaojiasuo represented higher drought resistance.The correlation analysis showed,that both the root length and the coleoptile length were highly correlated with D value(0.557 and 0.516),and they could be served as a sign in selecting for drought resistance of wheat.Thus,taking use of phenotype datasets and molecular markers,this work suggested a way to effectively evaluate the drought resistance of wheat during germination stage.
作者
张芳
颜安
任毅
杨卫君
耿洪伟
ZHANG Fang;YAN An;REN Yi;YANG Wei-jun;GENG Hong-wei(Key Laboratory of Agricultural Biological Technology,Xinjiang Agricultural University,Urumqi 830052;College of Pratacultural and Environmental Sciences,Xinjiang Agricultural University,Urumqi 830052)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第1期100-112,共13页
Journal of Plant Genetic Resources
基金
新疆农业大学-南京农业大学联合基金项目(KXYJ201602)~~
关键词
小麦
萌发期
抗旱性
隶属函数法
功能标记
wheat
germination stage
drought resistance
membership function
functional marker
