摘要
大百合是百合科大百合属一种重要的野生植物资源,本研究采用基因测序法,利用叶绿体DNA rpl16序列对10个大百合居群进行了遗传多样性和遗传结构分析,旨在为其资源保护和开发利用提供理论基础。结果显示:rpl16序列矩阵长度为699 bp,共检测到14个变异位点和12个单倍型,单倍型多态性(Hd)为0.604;总的遗传多样性(HT)为0.660。该居群间遗传变异(71.53%)大于居群内遗传变异(28.47%),说明居群间变异是其居群的主要变异来源;居群间遗传分化很高(Gst=0.677,Nst=0.614,Fst=0.71531),基因流较低(Nm=0.0995)。失配分析结果表明种群在进化过程中曾经历过快速扩张,中性检验支持上述结果:Tajima′s D=-1.74368(P>0.05);Fu and Li′s D=-2.69366(P<0.05);Fu and Li′s F=-2.79896(P<0.05)。研究结果表明:大百合居群遗传多样性较高,居群间存在较高的遗传分化,主要与其生活史特征相关。基于得到的居群遗传信息,建议采取就地保护为主的保护策略。
Cardiocrinum giganteum belongs to the genus Cardiocrinum (Endl.) Lindl.(Liliaceae), which is an important wild plant resource.In this study, the genetic diversity and genetic structure of 10 C.giganteum populations were analyzed by using the rpl16 sequence of the chloroplast DNA in order to provide theoretical basis for its resource protection and utilization.By alignment of 699 bp sequence, 14 mutation sites and 12 haplotypes were identified, with the haplotype polymorphism (Hd) and the total genetic diversity (HT) of 0.604 and 0.660, respectively.The genetic variation among the population (71.5 3%) was higher than the intra-population genetic variation (28.47 %), indicating the population variation largely contributed by different populations.The genetic differentiation among the population was high (Gst = 0.677, Nst = 0.614, Fst = 0.71531) and the gene flow was low (Nm = 0.0995).Mismatch analysis revealed that the population had experienced rapid expansion during the evolution process, which was supported by the neutrality tests (Tajima's D = -1.74368 (P > 0.05);Fu and Li's D = -2.69366 (P < 0.05);Fu and Li's F = -2.79896 (P < 0.05)).The study revealed the abundant genetic diversity and differentiation of C.giganteum particularly among populations, which was mainly related to the characteristics of life history.Thus, this work suggested an in-situ conservation strategy being employed in C.giganteum.
作者
张晓芸
杨妙琴
徐英
张永洪
李洪飞
ZHANG Xiao-yun;YANG Miao-qin;XU Ying;ZHANG Yong-hong;LI Hong-fei(Life Science Department,Yunnan Normal University,Kunming 650500;Xingyi Third Middle School,Guizhou Province,Xingyi 562400;Institute of Botany,the Chinese Academy of Sciences,Beijing 100093;Forest tree seedling station in Beibei district,Chongqing 400717)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第1期199-206,共8页
Journal of Plant Genetic Resources
基金
国家自然科学基金(31460050)
国家级大学生创业创新项目(201710681013)~~
关键词
大百合
rpl16
遗传多样性
遗传结构
Cardiocrinum giganteum
rpl16
genetic diversity
genetic structure
