摘要
目的探讨SLE患者外周血程序性死亡受体(PD)-1^hiCXCR5^-CD4^+T细胞的表达及临床意义。方法流式细胞术检测21例SLE患者、16名健康人外周血PD-1^hiCXCR5^-CD4^+T细胞占CD4^+T细胞百分比;放射免疫法测定21例SLE患者血清抗dsDNA抗体水平,统计学分析采用t检验及Pearson相关分析。结果SLE患者外周血PD-1^hiCXCR5^-CD4^+TT细胞亚群占CD4^+T细胞的百分率为[(2.1±2.0)%,n=21]高于健康对照组[(0.3±0.3)%,n=16](t=2.959,P<0.01)];中重度活动期SLE患者外周血PT细胞占CD4^+T细胞的百分率[(3.0±2.0)%,n=12]高于轻或非活动期组[(1.0±1.4)%,n=9](t=2.574,P<0.05)及健康对照组[(0.3±0.3)%,n=16](t=5.149,P<0.01);SLE患者外周血PD-1^hiCXCR5^-CD4^+T细胞占CD4^+T细胞的百分率与SLEDAI呈正相关(r=0.475,P=0.0297);抗dsDNA抗体阳性组SLE患者PD-1^hiCXCR5^-CD4^+T细胞占CD4^+T细胞的百分率[(2.7±2.1)%,n=15]高于抗dsDNA抗体阴性组[为(0.6±0.5)%,n=6](t=2.303,P<0.05);抗dsDNA抗体阳性组SLE患者PD-1^hiCXCR5^-CD4^+T细胞占CD4^+T细胞的百分率与抗dsDNA抗体滴度呈正相关(r=0.518,P=0.048)。结论SLE患者外周血PD-1^hiCXCR5^-CD4^+T细胞占CD4^+T细胞的百分率显著增加并与SLEDAI及抗dsDNA抗体呈正相关;PD-1^hiCXCR5^-CD4^+T细胞表达异常可能在SLE免疫发病机制中起重要作用。
Objective To investigate the expression of peripheral programmed death (PD)-1^hiCXCR5^- CD4^+ T cells and its clinical significance in systemic lupus erythematosus (SLE). Methods Peripheral blood PD-1^hiCXCR5^-CD4^+ T cells from 21 SLE patients and 16 healthy controls were examined by flow cytometry. The levels of serum anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies were determined using immunoradiometric as-say. Data were analyzed with t test and Pearson's correlation test. Results The per-centages of PD-1^hiCXCR5^- cells within CD4^+ T cell were significantly higher in SLE patients [(2.1±2.0)%] compared to normal controls [(0.3±0.3)%] (t=2.959, P<0.01). The percentages of PD-1^hiCXCR5^- cells within CD4^+ T cells in moderate to severe active SLE patients (3.0±2.0)% was significantly increased compared to patients with mild or inactive (1.0±1.4)% (t=2.574, P<0.05) and normal controls (0.3±0.3)% (t=5.149, P<0.01). The percentages of PD-1^hiCXCR5^- cells within CD4^+ T cells from SLE patients were positively related with systemic lupus erythematosus disease activity index (SLEDAI) (r=0.475, P=0.0297). SLE patients in serum anti-dsDNA antibodies positive group (2.7±2.1)% displayed a higher percentage of PD-1^hiCXCR5^- cells within CD4^+ T cells than patients in serum anti-dsDNA antibodies negative group (0.6±0.5)% (t=2.303, P<0.05). The percentages of PD-1^hiCXCR5^- cells within CD4^+ T cells from SLE patients were positively correlated with anti-dsDNA antibody titers. Conclusion The percentages of PD-1^hiCXCR5^- cells within CD4^+ T cells from SLE patients are increased and are positively correlated with SLEDAI and anti-dsDNA antibody levels. Increased percentage of PD-1^hiCXCR5^- cells within CD4^+ T cells might play an important role in the pathogenesis of SLE.
作者
周士亮
徐婷
蔡明渊
庄丽稞
张璐
陈锦云
张佩荣
孙茹蓉
谢雯
马英淳
吴敏
Zhou Shiliang;Xu Ting;Cai Mingyuan;Zhuang Like;Zhang Lu;Chen Jinyun;Zhang Peirong;Sun Rurong;Xie Wen;Ma Yingchun;Wu Min(Department of Rheumatology and Immunology,the First People's Hospital of Changzhou (The Third Affiliated Hospital of Suzhou University),Jiangsu 213003,China)
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2019年第1期15-18,共4页
Chinese Journal of Rheumatology
基金
国家自然科学基金青年科学基金(81302585)
江苏省常州市卫计委重大科技项目(ZD201707,ZD201609).
