摘要
目的观察大鼠垂体组织在急性坏死性胰腺炎时病理改变及探讨垂体组织损伤机制。方法24只SD大鼠,随机数字法分为正常组(normal group,N组)、假手术组(Sham operation group,SO组)、急性坏死性胰腺炎组(acute necrotizing pancreatitis group,ANP组),每组8只大鼠,逆行胆胰管注射5%牛磺胆酸钠制备(acute necrotizing pancreatitis,ANP)模型。全自动生化仪检测血清淀粉酶(amylase,AMY)、脂肪酶(lipase,LIP)水平;放射免疫分析法测定血清生长激素(growth hormone,GH)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、促甲状腺激素(thyroid stimulating hormone,TSH)及促性腺激素(follicle-stimulating hormone,FSH)水平;光镜下观察胰腺组织、垂体组织病理学改变;免疫组化法测定垂体组织半胱氨酸天冬酶-3(Caspase3)、半胱氨酸天冬酶-12(Caspase12)和C/EBP同源蛋白(CHOP)水平。结果与SO组比较,ANP组血清AMY(8679.16±307.60)U/L、LIP(9376.83±380.92)U/L水平明显升高,差异有统计学意义(P<0.05);ANP组血清ACTH(0.92±0.41)pg/ml、TSH(0.14±0.06)pg/ml、FSH(2.01±0.38)pg/ml水平明显降低,差异有统计学意义(P<0.05);ANP组垂体组织Caspase3(65.66±7.58)、Caspase12(70.66±4.76)和CHOP(143.16±19.05)水平明显增加,差异有统计学意义(P<0.05);光镜下ANP组胰腺损伤程度较SO组明显加重,差异有统计学意义(P<0.05),ANP组垂体组织充血程度较SO组明显加重。结论ANP时,大鼠垂体组织存在病理和功能改变,内质网应激损伤在垂体损伤中起到一定作用。
Objective To observe the pathological changes of pituitary tissue in rats with acute necrotizing pancreatitis and to explore the mechanism of pituitary tissue injury in rats. Methods 24 SD rats were randomly divided into normal group (N group, n=8) , sham operation group (SO group, n=8) , and acute necrotizing pancreatitis group (ANP group, n=8) . ANP model was established by retrograde injection of 5% sodium taurocholate into the biliopancretic duct. The serum levels of amylase (AMY) and lipase (LIP) were detected by automatic biochemical. The serum levels of growth hormone (GH) , adrenocorticotropic hormone (ACTH) , thyroid stimulating hormone (TSH) and follicle-stimulating hormone (FSH) were measured by radioimmunoassay. The pathological changes of pancreatic tissue and pituitary tissue were observed by the light microscope. The expression of Casepase3, Caspase12 and CHOP in pituitary tissue were determined by immunohistochemical method. Results Compared to SO group, the serum levels of AMY (8679.16±307.60) U/L and LIP (9376.83±380.92) U/L were significantly higher in ANP group (P<0.05) . The serum levels of ACTH (0.92±0.41) pg/ml, TSH (0.14±0.06) pg/ml, and FSH (2.01±0.38) pg/ml were significantly lower in ANP group (P<0.05) . The expression of Caspse 3 (65.66±7.58) , Caspase12 (70.66±4.76) and CHOP (143.16±19.05) in pituitary tissue were significantly increased in ANP group (P<0.05) . The pancreatic injury was more severe in ANP group under light microscope (P<0.05) . The degree of hyperemia of pituitary tissue of ANP group was aggravated. Conclusion Pathological changes occur in rat pituitary tissues and endoplasmic reticulum stress injury plays a role in pituitary injury during ANP.
作者
邓文宏
郭闻一
何小波
梅方超
洪育蒲
杨晓佳
王卫星
Deng Wenhong;Guo Wenyi;He Xiaobo;Mei Fangchao;Hong Yupu;Yang Xiaojia;Wang Weixing(Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China)
出处
《中华内分泌外科杂志》
CAS
2018年第6期478-482,共5页
Chinese Journal of Endocrine Surgery
基金
国家自然科学基金(81500488).
