摘要
以沙芥和斧形沙芥为试材,采用盆栽控水干旱法,研究不同干旱胁迫强度对其根和叶的抗坏血酸含量及其代谢相关酶活性的影响,探讨沙芥属植物抗坏血酸代谢对干旱胁迫的响应机制,揭示沙芥属植物抗坏血酸代谢方面的抗旱机理,评价沙芥和斧形沙芥抗旱性强弱。实验结果表明:随着干旱胁迫强度的加剧,沙芥和斧形沙芥的根和叶中抗坏血酸(ASA)、总抗坏血酸(T-ASA)含量均先增加后减少,脱氢抗坏血酸(DHA)、谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)以及总谷胱甘肽(T-GSH)含量逐渐增加, ASA/DHA、GSH/GSSG比值逐渐降低; ASA合成途径的L-半乳糖脱氢酶(GalDH)和L-半乳糖-1,4-内酯脱氢酶(GalLDH)以及再生途径的脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)、抗坏血酸氧化酶(AAO)、抗坏血酸过氧化物酶(APX)活性均先升高后降低。不同干旱胁迫下斧形沙芥根和叶中ASA含量、GalLDH、MDHAR活性以及叶中GSH含量、GalDH、GR活性和根中DHAR活性均高于沙芥,根中GSH含量、GalDH、AAO、GR活性和叶中DHAR、AAO活性均与沙芥相近,说明干旱胁迫下斧形沙芥能够维持较高的ASA合成和再生能力,其抗坏血酸代谢能清除更多的活性氧物质,抗旱性强于沙芥。
The Pugionium cornutum and P. dolabratum were chosen as the specimens and the potted water controlling and drying method was conducted to investigate the effects of drought stress on ascorbic acid contents and activities of metabolism related enzymes in roots and leaves, to discuss the response mechanism of ascorbic acid metabolism on drought stress, to explore the drought resisting mechanism in ascorbic acid metabolism, and to evaluate the drought resisting ability of two specimens. The results showed that the increasing intensification of drought stress led that the contents of ascorbic acid(ASA) and total ascorbic acid(T-ASA) were increased initially and decreased subsequently, the contents of dehydroascorbic acid(DHA), glutathione(GSH), oxidized glutathione(GSSG) and total glutathione(T-GSH) were increased gradually, however, both the ratio of ASA/DHA and GSH/GSSG were decreased. Besides, the activities of L-galactose dehydrogenase(GalDH), L-galactono-1,4-lactone dehydrogenase(GalLDH) in the ASA biosynthetic pathway, and dehydroascorbate reductase(DHAR), monodehydroascorbate reductase(MDHAR), ascorbic acid oxidase(AAO), and ascorbate peroxidase(APX) in roots and leaves during the ASA regeneration pathway were increased initially and decreased gradually. In addition, the content of ASA and the activities of GalLDH, MDHAR in roots and leaves, the content of GSH, the activities of GalDH, GR in leaves and the DHAR in roots of P. dolabratum were higher than those in P. cornutum. Furthermore, the content of GSH and the activities of GalDH, AAO and GR in roots, the DHAR, AAO in leaves of P. dolabratum were close to that in P. cornutum. Furthermore, the results also indicated that comparing with P. cornutum, the P. dolabratum could maintain the higher ASA biosynthesis ability and the higher regeneration capacity, the ascorbic acid metabolism in P. dolabratum could remove more reactive oxygen species, and also the drought resistance of P. dolabratum was stronger.
作者
郑清岭
杨忠仁
张晓艳
张凤兰
郝丽珍
ZHENG Qing-Ling;YANG Zhong-Ren;ZHANG Xiao-Yan;ZHANG Feng-Lan;HAO Li-Zhen(College of Agronomy Inner Mongolia Agricultural University,Inner Mongolia Key Laboratory of Germplasm and Germplasm Enhancement of Wild and Special Vegetable Inner Mongolia Agricultural University,Hohhot 010019,China;College of Agricultural Engineering,Nanyang Normal University,Henan Engineering Technology Research Center of Fungi Food,Nanyang,Henan 473061,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2018年第12期1865-1874,共10页
Plant Physiology Journal
基金
国家自然科学基金项目(31760570、31101541、31160393)
公益性行业(农业)科研专项(201203004)
内蒙古自然基金(2018MS03053).
关键词
沙芥
斧形沙芥
根
叶
干旱胁迫
抗坏血酸代谢
Pugionium cornutum
Pugionium dolabratum
roots
leaves
drought stress
ascorbic acid metabolism
