摘要
目的探讨三氯乙烯药疹样皮炎(OMDT)与人疱疹病毒6型(HHV6)、人巨细胞病毒(HCMV)既往感染、再激活、近期感染的关联。方法采用道抽样法,以20例OMDT患者为病例组,以20名职业接触三氯乙烯超过半年的健康人员为接触组,以20名无三氯乙烯接触史的健康人员为对照组。采用酶联免疫吸附测定方法测定3组人员血清中HHV6、HCMV免疫球蛋白(Ig) G、Ig M抗体,并采用聚合酶链式反应方法测定其全血DNA样本中HHV6和HCMV DNA。分析3组人群间、不同临床类型OMDT患者间HHV6和HCMV的既往感染率、再激活率、近期感染率的差异。结果病例组人群HHV6、HCMV既往感染率均高于对照组(65. 5%vs 20. 0%,75. 0%vs 15. 0%,P <0. 017)。病例组人群HHV6、HCMV再激活率虽高于对照组,但差异无统计学意义(P> 0. 017)。病例组人群HHV6和HCMV近期感染率分别与对照组比较,差异均无统计学意义(P> 0. 017)。接触组和对照组人群间、不同临床类型OMDT患者间HHV6和HCMV的既往感染率、再激活率、近期感染率分别比较,差异均无统计学意义(P> 0. 05)。结论 OMDT可能与HHV6、HCMV既往感染有关。
Objective To explore the potential association between occupational medicamentosa-like dermatitis induced by trichloroethylene( OMDT) and past infection,reactivation and recent infection of human herpesvirus 6( HHV6) and human cytomegalovirus( HCMV). Methods Twenty OMDT patients were recruited as case group by using judgment sampling method. Twenty healthy workers occupationally exposed to trichloroethylene for more than half a year were randomly selected as exposure group. Twenty healthy people with no exposure history to trichloroethylene were randomly selected as control group. The enzyme linked immunosorbent assay was used to qualitatively determine the titer of HHV6 and HCMV immunoglobulin( Ig) G,Ig M antibodies from serum samples of these subjects. The polymerase chain reaction was used to qualitatively detect HHV6 and HCMV DNA from whole blood DNA samples of these subjects. The differences of previous infection rate,reactivation rate and recent infection rate of HHV6 and HCMV among these three groups of patients with different clinical types of OMDT were analyzed. Results The prevalence of HHV6 and HCMV infection in the case group was higher than that in the control group,and the difference was statistically significant( 65. 5% vs 20. 0%,75. 0% vs15. 0%,P < 0. 017). The reactivation rate of HHV6 and HCMV in the case group was higher than that in the control group,but the difference was not statistically significant( P > 0. 017). The recent infection rate of HHV6 and HCMV in the case group was not significantly different from that in the control group( P > 0. 017). There was no significant difference in the past infection rate,reactivation rate and recent infection rate of HHV6 and HCMV between the exposure group and the control group( P > 0. 05),meanwhile in different clinical types of OMDT patients( P > 0. 05). Conclusion OMDT may be associated with past infection of HHV6 and HCMV.
作者
林大枫
王佃鹏
刘慧敏
谢家伟
张艳芳
黄先青
LIN Dafeng;WANG Dianpeng;LIU Huimin;XIE Jiawei;ZHANG Yanfang;HUANG Xianqing(Clinical Laboratory of Shenzhen Prevention and Treatment Center for Occupational Diseases & Effect Laboratory of Poison Detection Center,Shenzhen ,Guangdong 518010,China)
出处
《中国职业医学》
CAS
北大核心
2018年第6期686-690,共5页
China Occupational Medicine
基金
国家自然科学基金青年科学基金项目(81502797)
深圳市科创委学科布局项目(JCYJ20160429090813380)
关键词
三氯乙烯
药疹
皮炎
人疱疹病毒6型
巨细胞病毒
感染
激活
Trichloroethylene
Medicamentosa
Dermatitis
Human herpesvirus 6
Human cytomegalovirus
Infection
Activation
