摘要
目的:建立HPLC同时测定陈香露白露片中甘草苷、芸香柚皮苷和橙皮苷含量的方法,以提高陈香露白露片的质量标准。方法:采用Phenomenex Gemini C18柱(4.6 mm×250 mm, 5μm),以乙腈-0.2%乙酸(19:81)为流动相,检测波长277 nm,体积流量为1 mL·min-1,柱温30℃。结果:甘草苷、芸香柚皮苷和橙皮苷线性范围分别为0.025 6～0.818 2μg (r=0.999 6),0.026 4～0.8448μg (r=0.9995),0.1275～4.0788μg (r=0.9996);平均回收率分别为97.78%(RSD1.58%),97.65%(RSD1.31%),98.01%(RSD 1.17%)。结论:建立的陈香露白露片含量测定方法操作简便,准确度高,提高了产品检验的工作效率,可有效控制产品质量。
OBJECTIVE To improve the quality standard of Chenxiang Lubailu tablets, detect. the contents of liquiritin,narirutin and hesperidin in the prescription simultaneously by HPLC. METHODS Phenomenex Gemini C18(4.6 mm × 250 mm, 5 μm) column was used, with the mobile phase consisting of acetonitrile and 0.2% acetic acid(19:81), the DAD-3000 detection wavelength set at 277 nm, the flow rate at 1 mL·min-1, and the column temperature at 30 ℃. RESULTSUnder the chromatographic conditions, the linear ranges of liquiritin, narirutin and hesperidin were 0.025 6-0.818 2 μg(r= 0.999 6), 0.026 4-0.844 8 μg(r = 0.999 5) and 0.127 5-4.078 8 μg(r = 0.999 6), respectively. The average recoveries were 97.78%(RSD 1.58%), 97.65%(RSD 1.31%) and 98.01%(RSD 1.17%), respectively. CONCLUSION The detection method for the components in the Chenxiang Lubailu tablets is simple, accurate, and can improve the work efficiency and,effectively control the product quality.
作者
冯发进
陆宽
冯乾乾
杜成兴
杨熟英
杨迺嘉
霍昕
FENG Fa-jin;LU Kuan;FENG Qian-qian;DU Cheng-xing;YANG Shu-ying;YANG Nai-jia;HUO Xin(Guizhou Institute of Biotechnology Research and Development,Guizhou Guiyang 550002,China;Kehui Pharmaceutical Factory in Guizhou Province,Guizhou Qingzhen 551400,China)
出处
《中国医院药学杂志》
CAS
北大核心
2019年第2期116-119,共4页
Chinese Journal of Hospital Pharmacy
基金
贵州省科学技术项目(编号:黔科合条[2015]002号)
贵州省科技计划项目(编号:黔科合开发字[2017]01号)
