摘要
光遗传学实验技术是一种通过联合使用光学技术和遗传工程技术实现对特定细胞功能进行精确干预的变革性研究手段,是近几十年来神经生物学领域最大的突破技术之一。但是该技术涉及多学科交叉的理论知识和方法,而且实验周期长,设备昂贵,除了仿真教学外不便于在本科生实验课堂上广泛开展。在本虚拟仿真实验中,通过仿真病毒包装过程,仿真动物选择、手术及病毒立体定位微注射过程,仿真光敏通道表达情况的观察过程,仿真光敏通道功能在体细胞外的验证过程,仿真光遗传抑制杏仁核谷氨酸能神经元影响动物恐惧行为的观察过程,学习光遗传学技术的基本原理、操作与应用,实验教学效果理想。本文对该虚拟实验的脚本制作内容设计作一探讨。
Optogenetics is one of the biggest breakthroughs in neurobiology in recent decades, which is a revolutionary approach to precise intervention of specific cell functions through the combination of optical and genetic engineering techniques. However, this technique involves the interdisciplinary theoretical knowledge and methods, and it requires expensive equipments and long experimental period. Thus, it is not convenient to carry out widely in experiment teaching for undergraduates except the virtual simulation teaching. In present virtual simulation experiment, we aim to learn the basic principle, operation and application of optogenetics and achieve the desired teaching effects by simulating the process of the virus package, simulating the process of the animal selection, surgery and virus stereotactic microinjection, simulating the expression process of photosensitive channel, the extracellular verification process of photosensitive channel function in vivo, and simulating the observation process that optogenetic inhibition of glutamate neurons in the amygdala affect animal fear behavior. In this paper, the content design of the script making of the virtual experiment has been discussed in the above ways.
作者
吴广延
姚娟
隋建峰
叶建宁
Wu Guangyan;Yao Juan;Sui Jianfeng;Ye Jianning(Experimental Center of Basic Medicine,College of Basic,Medical Sciences,Army Medical University, Chongqing 400038,China;Department of Neurology,the Second Affiliated Hospital of Army Medical University,Chohgqing 400037,China)
出处
《中华医学教育探索杂志》
2019年第1期58-61,共4页
Chinese Journal of Medical Education Research
基金
国家自然科学基金面上项目(81571161、81771213)
国家自然科学基金青年项目(31700913).
关键词
虚拟仿真实验
实验教学
光遗传学
脚本
Virtual simulation experiment
Experimental teaching
Optogenetics
Script
