摘要
以海参斑软骨为原料,采用水提醇沉法得到粗多糖,通过Sevag法除去蛋白,采用DEAE-52纤维素和葡聚糖G-75纯化得到组分均一的3种多糖FCPS-1、FCPS-2、FCPS-3。对3种多糖进行了化学成分分析及紫外波长扫描,使用天青A比色法对3种多糖进行了肝素效价测定,利用FTIR和NMR对FCPS-3进行了结构表征,采用GPC测定了FCPS-3的相对分子质量并测定其体外抗氧化活性。结果显示:3种多糖总糖质量分数均超过90%,蛋白质量分数低于3%。紫外波长扫描显示,3种多糖在260nm以及280nm处紫外吸收峰较弱,表明3种多糖中蛋白质及核酸含量较低。天青A显色结果显示,FCPS-3肝素效价最高,达到1.365 kU/g;红外及核磁波谱显示,FCPS-3为酸性多糖,且含有硫酸根,为β型吡喃糖。GPC测定FCPS-3的Mw为175700 Da,Z均相对分子质量为253032Da,Mn为121885Da。当FCPS-3质量浓度为5g/L时,其对DPPH?、ABTS+?以及OH?的清除率分别为78.63%、96.26%、93.15%。
Crude polysaccharides of lumpfish cartilage were obtained by water extraction and alcohol precipitation,and then Sevag method was used for deproteinization.The purification of crude polysaccharides was carried by DEAE-52 cellulose and Sephadex G-75,and three kinds of polysaccharides,FCPS-1,FCPS-2 and FCPS-3 were obtained.These three polysaccharides composition were analyzed and characterized by UV.Heparin titer of three polysaccharides was measured by azure A colorimetric method.The structure of FCPS-3 was characterized by FTIR and nuclear magnetic resonance(NMR) spectroscopy.Moreover,the molecular weight of FCPS-3 was determined by GPC and its antioxidant activity was measured in vitro.The results showed that the mass fraction of three polysaccharides all exceeded 90%,the mass fraction of protein was less than 3%.The UV absorption spectra showed that two weak characteristic absorption peaks of three polysaccharides were observed at 260 nm and 280 nm,indicating that the content of protein and nucleic acids in three polysaccharides was very low.The heparin titer of FCPS-3 was the highest,reaching 1.365 U/mg.FTIR and NMR results revealed that FCPS-3 was acid polysaccharides and contained sulfate as β-glucopyranose.The weight average molecular weight(Mw) of FCPS-3 was 175700 Da,the Z average molecular weight was 253032 Da,and the number average molecular weight(Mn) was 121885 Da.When the mass concentration of FCPS-3 was 5 g/L,its scavenging capabilities toward DPPH·,ABTS+·,OH· free radical was 78.63%,96.26% and 93.15%,respectively.
作者
郑捷
孙凯华
胡爱军
高建忠
孙朋朋
高芯
张芳
ZHENG Jie;SUN Kai-hua;HU Ai-jun;GAO Jian-zhong;SUN Peng-peng;GAO Xin;ZHANG Fang(State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology,Tianjin 300457, China;Tianjin Hongteng Aquatic Science &Technology Development Co.Ltd.,Tianjin 301800,China;Tianjin Jinbaodi Manor Food Co.Ltd.,Tianjin 301800,China;College of Food Engineering and Biotechnology,Tianjin University of Science &Technology,Tianjin 300457,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2019年第1期66-73,共8页
Fine Chemicals
基金
2016年科技小巨人领军企业培育重大项目(16YDLJNC00170)
2017年一二三产业融合发展科技示范工程项目(17ZXYENC00140)~~
关键词
软骨多糖
纯化
结构
肝素效价
抗氧化
生物工程
cartilage polysaccharide
purification
structure
heparin titer
antioxidation
biological engineering
