摘要
以脱氮假单胞菌为出发菌株,利用化学诱变剂-亚硝基胍(NTG)结合抗生素抗性筛选以提高维生素B_(12)的生产水平。通过对致死率和正突变率的考察,确定NTG浓度为25μg/mL,利福平和庆大霉素的最适筛选浓度分别为4.5μg/mL和35μg/mL。得到的突变株经摇瓶发酵后,采用高效液相色谱仪(HPLC)对最终发酵液进行检测。筛选到的突变株RE-24最高产量达到243.3μg/mL,比原始菌株提高了33.5%。
Chemical mutagenesis(NTG)and resistance selection was used to increase the production of vitamin B12 from original strain of Pseudomonas denitrificans.The results showed that the dose of NTG mutagenesis was determined 25μg/mL in term of lethality rate and positive mutation.The minimum inhibitory concentration(MIC)of the Rifampicin and Gentamicin were 4.5μg/mL & 35μg/mL.The final broth in flasks was determined by HPLC.High vitamin B12-producing mutant RE-24 was isolated in the screening experiments,and its yield of vitamin B12 reached 243.3μg/mL,which was 33.5% higher than its parental strain.
作者
韩龙
阮国祥
马杰希
刘伟
HAN Long;RUAN Guo-xiang;MA Jie-xi;LIU Wei(Shandong Hongda Biological Technology Company,Linyi 276400,China)
出处
《精细与专用化学品》
CAS
2019年第1期36-39,共4页
Fine and Specialty Chemicals
关键词
维生素B12
化学诱变
抗性筛选
vitamin B12
chemical mutagenesis
antibiotic resistance
