摘要
目的检测并探讨microRNA-218在前列腺癌组织与血清中表达及相关性。方法收集72例初次诊断为前列腺癌患者(PCa)、80例前列腺增生患者(BPH)的组织与相应术前血清,86例激素依赖性前列腺癌患者(ADPC)、76例激素难治性前列腺癌患者(HRPC)血清。临床资料通过回顾分析患者住院期间病历资料获得。采用实时荧光定量法检测组织与血清中microRNA-218的表达情况,分析其在前列腺组织与血清中表达的相关性,与临床、病理特性及前列腺癌进展的相关性。结果前列腺增生患者组织及相应血清中microRNA-218的表达水平均高于前列腺癌患者(P <0. 001)。前列腺增生患者、前列腺癌患者组织与血清中microRNA-218的表达均呈正相关(r=0. 815,0. 423,P <0. 001)。初诊前列腺癌组织、血清中microRNA-218的表达水平与总前列腺特异性抗原(t PSA)、临床分期、病理分级均呈负相关(P <0. 05)。血清microRNA-218在激素依赖性前列腺癌患者与初诊前列腺癌患者中表达差异无统计学意义(P> 0. 05),但显著低于激素难治性前列腺癌患者(P <0. 05),且均与t PSA呈正相关(P <0. 05)。结论前列腺增生及前列腺癌患者血清microRNA-218表达水平变化可反映组织中microRNA-218的表达水平变化情况。血清MicroRNA-218有望成为一个前列腺癌早期诊断、前列腺癌进展中有潜在价值的生物标志物。
Objective To investigate and explore expression status of microRNA-218,and their correlation in serum and tissue samples of prostate cancer patients.Methods The serum and tissue samples of 72 patients in first diagnosised prostate cancer (PCa)and 80 benign prostate hyperplasia (BPH)patients were collected.Serum samples of 162 patients,including 86 patients with androgen-dependent prostate cancer (ADPC)and 76 patients with hormone-refractory prostate cancer (HRPC)were collected.The expression level of microRNA-218 in the tissue and serum samples were detected by specific SYBR Green quantitative real-time polymerase chain reaction (PCR).The correlation between the serum microRNAs and tissue microRNAs expressions tatus were analyzed,and their relationship with clinical characterization were also analyzed.Results The expression level of microRNA-218 in tissues and serum of patients with BPH was higher than that of patients with PCa (P <0.001 ).MicroRNA-218 level in PCa and BPH tissue were both correlated with that in the serum of PCa and BPH (rBPH =0.815,rPCa =0.423 ).The expression level of microRNAs in tissue and serum samples from PCa were negatively correlated to total prostate specific antigen (tPSA),clinical stages and pathological grade (P <0.05).Serum microRNA-218 expression was not significantly different between hormone-dependent PCa patients and newly diagnosed PCa patients,but was significantly lower than that of HRPC patients (P <0.05),and was positively correlated with tPSA (P < 0.05).Conclusions The changes of microRNA-218 expression in serum of patients with BPH and prostate cancer can reflect the changes of microRNA-218 expression in tissues.Serum microRNA-218 is expected to be a potential biomarker for early diagnosis and progression of prostate cancer.
作者
王磊
程广舟
阳东荣
刘媛媛
张庆磊
张文宇
Wang Lei;Cheng Guangzhou;Yang Dongrong;Liu Yuanyuan;Zhang Qinglei;Zhang Wenyu(Department of Urology,Tengzhou Central People's Hospital,Tengzhou 277500,China;Department of Urology,The Second Affiliated Hospital of Soochou University,Suzhou 215000,China;Department of Neurology,Tengzhou Central People's Hospital,Tengzhou 277500,China;Center for Systems Biology,Soochow University Central lab,Suzhou 215006,China)
出处
《中国医师杂志》
CAS
2019年第1期76-80,共5页
Journal of Chinese Physician
关键词
微RNAS
前列腺肿瘤
肿瘤
激素依赖性
前列腺增生
MicroRNAs
Prostatic neoplasms
Neoplasms,hormone-dependent
Prostatic hyperplasia
