摘要
以芥蓝(Brassicaoleraceavar.alboglabra)为材料,以ζ–胡萝卜素脱氢酶(ζ-Carotene desaturase,ZDS)基因为目标基因,建立其CRISPR/Cas9基因组编辑体系。在BoaZDS的编码区近5′端选择靶位点,构建了CRISPR/Cas9表达载体,通过农杆菌介导的遗传转化方法获得了19个芥蓝转基因阳性植株,Sanger测序分析发现其中13株成功突变,CRISPR/Cas9载体在芥蓝上的突变效率为68.42%,且所有突变植株均表现出明显的白化表型。
The ζ-carotene desaturase(ZDS)gene was selected as the target gene to establish the CRISPR/Cas9 genome editing system in Chinese kale. The target site,near the 5′ end of the coding region of the BoaZDS,was selected,and the CRISPR/Cas9 expression vector was constructed. As a result,a total of 19 transgenic plants were obtained through Agrobacterium-mediated genetic transformation. Sanger sequencing analysis revealed that 13 of them were mutated. The mutation efficiency was 68.42%,and all mutants showed a clear albino phenotype.
作者
郑爱红
张芬
江敏
袁巧
江雷雨
陈清
汤浩茹
孙勃
ZHENG Aihong;ZHANG Fen;JIANG Min;YUAN Qiao;JIANG Leiyu;CHEN Qing;TANG Haoru;and SUN Bo(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2019年第1期57-64,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31500247)
四川省教育厅重点项目(14ZA0016)
国家级大学生创新训练计划项目(201710626030)
