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响应面法优化猪血复合微量元素补铁剂螯合工艺 被引量:3

Optimization of Iron Chelating for Pig Blood Derived Composite Iron Supplement Containing Multiple Trace Elements by Response Surface Methodology
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摘要 采用响应面法优化猪血复合微量元素补铁剂的螯合工艺。在单因素试验的基础上,选择pH值、多肽溶液质量分数、多肽溶液与1.0 mol/L FeCl_2溶液的体积比为影响因素,以Fe^(2+)螯合率为指标,进行3因素3水平的Box-Behnken试验设计,采用响应面法分析3个因素对响应值的影响。结果表明:响应面法优化猪血复合微量元素补铁剂的最佳螯合工艺条件为p H值5.40、多肽溶液质量分数2.27%、多肽溶液与1.0 mol/L FeCl_2溶液的体积比2.16∶1;在此条件下,猪血多肽Fe^(2+)螯合率的预测值为79.37%,实测值为79.41%。 The objective of this study was to optimize the chelating process for composite microelement iron supplement prepared from pig blood by response surface methodology. By using one-factor-at-a-time method, pH value, peptide concentration, and volume ratio of peptide solution to FeCl2 solution(1.0 mol/L) were identified as factors that significantly influence Fe^2+ chelating efficiency. Subsequently, three-dimensional response surface plots were generated using a threevariable three-level Box-Behnken design to investigate the interaction among the three factors. The optimized conditions were established as follows: pH 5.40, peptide concentration 2.27%, and volume ratio of peptide solution to FeCl2 solution(1.0 mol/L) 2.16:1. Under these conditions, the predicted Fe2+ chelating efficiency was 79.37%, and the actual data was 79.41%.
作者 黄晶晶 张福生 鄢嫣 殷俊峰 周迎芹 谢宁宁 HUANG Jingjing;ZHANG Fusheng;YAN Yan;YIN Junfeng;ZHOU Yingqin;XIE Ningning(Institute of Agricultural Products Processing,Anhui Academy of Agricultural Sciences,Hefei 230031,China)
出处 《肉类研究》 北大核心 2018年第12期24-30,共7页 Meat Research
基金 国家自然科学基金青年科学基金项目(31801673) 安徽省农业科学院人才发展专项(17F1205) 安徽省农业科学院院长青年创新基金项目(17B1220) 安徽省农业科学院院所共建团队项目(18C1225) 安徽省自然科学基金青年科学基金项目(1808085QC94)
关键词 响应面法 猪血蛋白 补铁剂 Fe^2+螯合率 response surface methodology pig blood protein iron supplement Fe^2+chelating rate
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