摘要
黄花蒿Artemisia annua又名青蒿,是中国传统中药,其有效成分青蒿素是一种含过氧桥基团结构的倍半萜内酯类化合物,是一种能有效抗疟的药物。很多理化因子例如盐分、水分、光照、植物激素等均能诱导黄花蒿中次生代谢产物青蒿素的合成,温度作为一种重要的生长因素对青蒿素的合成也有极大地影响。该文旨在研究高温诱导对黄花蒿中青蒿素生物合成的影响。将黄花蒿幼苗放置在25,40℃条件下,分别在0,3,12,36 h后取样,利用液相色谱-质谱联用法测定各个样品中的青蒿素含量;提取样品的总RNA,进行转录组测序并且利用实时荧光定量PCR技术定量分析青蒿素合成途径及竞争途经关键酶基因的表达。结果显示40℃处理3,12,36 h后,青蒿素质量分数分别提高20%,42%,68%;FDS,ALDH1,CYP71AV1和ADS的表达量分别上调4. 3,3. 3,2. 5,1. 9倍,SQS和BPS的表达量下调了37%和90%。综上,高温可以通过促进青蒿素合成途径合成酶基因表达量,并且抑制青蒿素竞争途径合成酶基因的表达量从而促进青蒿素的生物合成。
Artemisia annua also known as Qinghao,is a kind of traditional Chinese medicine. Its active ingredient is artemisinin,a sesquiterpene lactone compound with a peroxy bridging group structure. A. annua is an effective antimalarial drug. Artemisinin,a secondary metabolite in A. annua,can be induced by many physical and chemical factors,such as salinity,moisture,light,and plant hormones. Temperature,as an important growth factor,also has a great influence on the synthesis of artemisinin. This article aims to study the effect of high temperature on inducing artemisinin biosynthesis in A. annua. The A. annua seedlings were placed at 25,40℃,and the samples were taken after 0,3,12 and 36 h. The content of artemisinin in each sample was determined by liquid chromatography-mass spectrometry. Total RNA was extracted from the samples,and then transcriptome sequencing and real-time fluorescence quantitative PCR were used to quantitatively analyze the expression of the key enzyme genes in artemisinin synthesis pathway and competition pathway. The results showed that artemisinin content was increased by 20%,42% and 68% after 3,12,36 h of treatment at40 ℃. The expression levels of FDS,ALDH1,CYP71 AV1 and ADS were up-regulated by 4. 3,3. 3,2. 5,1. 9 times,and the expression levels of SQS and BPS were downregulated by 37% and 90% respectively. In summary,high temperature can promote the biosynthesis of artemisinin by promoting the expression of synthetase genes in artemisinin synthesis pathway and inhibiting the expression of synthetase genes in artemisinin-competition pathway.
作者
鹿江南
张栋
丁丹丹
高翰
韩宗贤
刘霞
向丽
LU Jiang-nan;ZHANG Dong;DING Dan-dan;GAO Han;HAN Zong-xian;LIU Xia;XIANG Li(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan 430070,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第20期4169-4176,共8页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2017ZX09101002-003-001)
港澳台科技合作项目(2015DFM30030)
关键词
黄花蒿
青蒿素
高温诱导
转录组
基因表达
Artemisia annua
artemisinin
high temperature induction
transcriptome
gene expression
