摘要
目的:观察隔药灸改善UC大鼠结肠炎症的效应,通过NF-κB通路和STAT3磷酸化参与免疫调节的作用机制。方法:将大鼠随机分成正常组、UC组和隔药灸组,每组8只。采用4%DSS诱导UC的大鼠模型,造模成功后采用隔药灸大鼠双侧天枢穴,每穴灸2壮艾炷,每天1次,共7次。通过比较组织病理学、血清学促炎症因子的蛋白浓度观察隔药灸干预UC大鼠的效应。采用Western Blot检测结肠组织NF-κB通路及STAT3活性探讨隔药灸改善UC大鼠结肠炎症反应的潜在机制。结果:UC组大鼠结肠组织病理学提示黏膜上皮表面溃疡形成,炎症反应明显,隔药灸能修复结肠上皮损伤,减轻UC大鼠结肠组织的炎症反应。与正常组比较,UC组大鼠血清IL-1β和IL-6蛋白浓度显著升高(P <0.05,P <0.01),p-STAT3磷酸化水平显著增加,pIκB-α和NF-κB p65蛋白表达均显著增加,IκB-α蛋白表达显著降低;与UC组比较,隔药灸组大鼠血清IL-1β和IL-6蛋白浓度显著降低(P <0.05),结肠组织NF-κB p65蛋白表达和pIκB-α磷酸化水平均降低,IκB-α蛋白表达增加。结论:隔药灸可能通过抑制NF-κB通路和STAT3磷酸化,减少了促炎症因子IL-1β和IL-6的释放,从而减轻了UC大鼠的结肠炎症反应。
Objective: To observe the effect of herb-partition moxibustion on colonic inflammation in UC rats, and participate in the regulation of immune regulation via NF-κB pathway and STAT3 phosphorylation. Methods: Rats were randomly divided into normal group, the ulcerative colitis group(UC) group and herb-partition moxibustion group, with 8 rats in each group. The rat model of UC was induced by 4% DSS. After successful modeling, the rats were treated with moxibustion on bilateral Tianshu acupoints(ST25). Each acupoint was used with 2 Zhuang moxa,1 time a day, for 7 times. The effect of the herb-partition moxibustion on UC rats was observed by comparing the histopathological and protein concentrations of serum proinflammatory cytokine. Western Blot was used to detect NF-κB pathway and STAT3 activity in colon tissue. Results: Colonic histopathology in the UC group showed that the mucosal epithelium with ulcer formation and obvious inflammatory response. The herb-partition moxibustion could repair colonic epithelial damage and reduce the inflammatory response of colon tissue in UC rats. Compared with the normal group, the serum IL-1β and IL-6 protein concentrations were significantly increased in the UC group(P < 0.05, P < 0.01), and the STAT3 phosphorylation level and protein expression levels of pIκB-α and NF-κB p65 were significantly increased. The protein expression level of IκB-α was significantly decreased. Compared with the UC group, the serum protein concentrations of IL-1β and IL-6 were significantly lower in the herb-partition moxibustion group(P < 0.05), and protein expression level of NF-κB p65 was decreased in the colon tissue. The phosphorylation level of pIκB-α was decreased, while the protein expression level of IκB-α was increased. Conclusion: Herb-partition moxibustion reduced colonic inflammatory response in UC rats by DSS-induced, the underlying mechanism may related to decrease release of proinflammatory cytokines IL-1β and IL-6 via dual inhibition of NF-κB and STAT3 activation.
作者
赵继梦
吴焕淦
陆嫄
顾沐恩
马喆
刘雅楠
郑寒丹
刘慧荣
马晓芃
黄艳
Zhao Jimeng;Wu Huangan;Lu Yuan;Gu Muen;Ma Zhe;Liu Yanan;Zheng Handan;Liu Huirong;Ma Xiaopeng;Huang Yan(Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第9期1579-1584,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家科技部重点基础研究发展计划"973计划"项目(2015CB554501):艾灸效应的启动机制及其内源性调节作用的机理研究
负责人:吴焕淦
国家自然科学基金委青年基金项目(81503659):基于组蛋白H3赖氨酸27修饰的针灸对溃疡性结肠炎IL-9R调节机制研究
负责人:黄艳
国家自然科学基金委面上项目(81574081):艾灸对结肠炎相关性癌变的ATP-P2X7R的作用及调控机制研究
负责人:吴焕淦
上海市卫计委科研项目资助面上项目(201640284):针灸对溃疡性结肠炎的组蛋白3赖氨酸3修饰的调节机制研究
负责人:黄艳
