摘要
目的:探讨汉黄芩素对髓母细胞瘤增殖和凋亡的影响。方法:运用CCK-8(cell counting kit-8)试剂盒,流式细胞仪检测不同浓度汉黄芩素及同一浓度不同作用时间对髓母细胞瘤细胞活性及凋亡的影响;通过比色法检测汉黄芩素对髓母细胞瘤细胞中Caspase-3蛋白活性的影响;通过体内试验,检测汉黄芩素在裸鼠成瘤实验中对髓母细胞瘤细胞活性的影响。结果:汉黄芩素可以抑制髓母细胞瘤细胞活性并诱导髓母细胞瘤细胞凋亡。汉黄芩素抑制髓母细胞瘤细胞活性具有浓度依赖性和时间依赖性。此外,汉黄芩素可增强髓母细胞瘤细胞中凋亡蛋白Caspase-3的活性,诱导髓母细胞瘤细胞凋亡。在裸鼠成瘤实验中,汉黄芩素可抑制髓母细胞瘤的体内增殖。结论:汉黄芩素在体外可抑制髓母细胞瘤细胞活性及诱导细胞凋亡。在裸鼠成瘤实验中,汉黄芩素可以抑制肿瘤增长。
Objective:To explore the impact of wogonin on the proliferation and apoptosis of medulloblastoma cell line DAOY.Methods:DAOY cells were divided into experimental groups(added with wogonin at difference concentrations)and control group. The proliferation of DAOY was detected using CCK-8 assay kit.The apoptosis was analyzed by Annexin Ⅴ-FITC/PI double staining and the activity of caspase-3 was determined via colorimetry. Moreover,we also detected the tumor inhibiting function of wogonin in vivo using subcutaneous tumor model. Results:Wognin remarkably inhibited the proliferation of medulloblastoma cell DAOY and induced the apoptosis in a dose and time dependent manner. The apoptosis was characterized by the increase of apoptosis protein caspase-3.Conclusion:Wogonin could inhibit medulloblastoma cell proliferation both in vitro and in vivo via inducing medulloblastoma cells apoptosis.
作者
王新法
何俊平
张瑞
钱静
王静
王刚
Wang Xinfa;He Junping;Zhang Rui;Qian Jing;Wang Jing;Wang Gang(Department of neurosurgery,Nanjing Children's Hospital ,Affiliated to NMU,Nanjing 210008,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第12期1688-1691,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科技发展基金面上项目(2017NJMU066)
关键词
髓母细胞瘤
汉黄芩素
增殖
凋亡
medulloblastoma
wogonin
proliferation
appoptosis
