摘要
目的研究转录因子缺氧诱导因子-1α(HIF-1α)在人卵巢癌组织中的表达水平,HIF-1α抑制剂3-(5-羟甲基-2-呋喃基)-1-苯甲基吲唑(YC-1)对人卵巢癌细胞A2780s的细胞凋亡及细胞周期的影响,及其与顺铂(CDDP)抗肿瘤治疗的协同作用。方法下载并分析可公开获取的基因表达综合(GEO)数据库,比较HIF-1α基因在上皮性卵巢癌和正常卵巢组织中的表达水平。将培养好的A2780s细胞随机分为空白组、对照组Ⅰ、对照组Ⅱ和实验组。空白组给予等体积的10%小牛血清RPMI培养液处理,对照组Ⅰ用10μmol·L^(-1)YC-1处理,对照组Ⅱ用10μg·L^(-1)顺铂处理,实验组用10μmol·L^(-1)YC-1和10μg·L^(-1)顺铂处理。以流式细胞仪测定细胞凋亡及细胞周期分布的变化。结果 HIF-1α基因在上皮性卵巢癌组织中的表达水平显著高于正常卵巢组织(P <0. 05)。对照组Ⅰ、对照组Ⅱ和实验组均诱导人卵巢癌A2780s细胞凋亡并使细胞周期阻滞于S期。对照组Ⅰ、对照组Ⅱ和实验组对卵巢癌细胞增殖的抑制率分别为(8. 80±0. 24)%,(14. 41±0. 75)%,(20. 52±1. 23)%,细胞S期阻滞率分别为(45. 77±0. 73)%,(49. 75±0. 66)%,(54. 43±0. 91)%,实验组与对照组Ⅰ、对照组Ⅱ比较,差异均有统计学意义(均P <0. 05)。结论 HIF-1α高表达于人卵巢癌; YC-1与顺铂联合应用治疗人卵巢癌可通过增强细胞凋亡和细胞周期阻滞发挥协同抗肿瘤作用。
Objective To investigate the expression level of transcription factor hypoxia inducible factor-1α( HIF-1α) in human and the inhibitory effect of 3-( 5’-hydroxymethyl-2’-furyl)-1-benzylindazole( YC-1) on cell cycle distribution and apoptosis of human oophoroma A2780 s cells,and its synergetic effect with cisplatin. Methods To download and analyze the publicly available Gene Expression Omnibus( GEO) database to compare the expression level of HIF-1α gene in epithelial ovarian cancer and normal ovarian tissue. The cultured A2780 s cells were randomly divided into four groups: blank group( treated with equal volume of RPMI 1640 medium with 10% fetal calf serum),controlⅠ( add 10 μmol·L^-1 YC-1),control Ⅱ( add 10 μg ·L^-1 cisplatin),experimental( add 10 μmol · L^-1 YC-1 + 10 μg · L^-1 cisplatin). The cell cycle distribution and the apoptosis rate of A2780 s cells were determined by flow cytometry( FCM). Results The expression of HIF-1α gene in epithelial ovarian cancer was significantly higher than that in normal ovarian tissue( P < 0. 05). Control Ⅰ,control Ⅱ and experimental groups were induced cell apoptosis and S phase arrest to inhibit proliferation in human oophoroma A2780 s cells. The inhibitory rates in control Ⅰ, control Ⅱ and experimental groups were( 8. 80 ± 0. 24) %,( 14. 41 ± 0. 75) %,( 20. 52 ± 1. 23) %,the S phase cell distribution were( 45. 77 ± 0. 73) %,( 49. 75 ± 0. 66) %,( 54. 43 ± 0. 91) %. There was significant difference between experimental group and control Ⅰand control Ⅱ gorups( P < 0. 05). Conclusion HIF-1α expression was elevated in human oophoroma;YC-1 and cisplatin can synergistically suppress human oophoroma cells through enhanced cell apoptosis and cell cycle arrest.
作者
周筠
洪莉
黄金玲
洪莎莎
ZHOU Yun;HONG Li;HUANG Jin-ling;HONG Sha-sha(Department of Obstetrics and Gynecology,Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2019年第2期137-139,共3页
The Chinese Journal of Clinical Pharmacology
基金
湖北省自然科学基金资助项目(2010CDB06903)
