摘要
目的检测胰腺癌组织中microRNA-7的表达,分析其作为胰腺癌诊断的临床价值。方法荧光定量PCR检测53例胰腺癌及癌旁组织和75例胰腺良性病变者microRNA-7的表达量,探讨其在胰腺癌组织和良性疾病中的表达水平差异,并利用统计学分析其与胰腺癌各临床病理特征之间的联系。结果胰腺癌组织中microRNA-7相对表达量低于癌旁组织组及胰腺良性病变组,差异有统计学意义(P<0.01)。Ⅲ期+Ⅳ期患者中microRNA-7水平显著低于Ⅰ期+Ⅱ期患者(P<0.01);淋巴结转移者较未转移者显著降低,差异均有统计学意义(P<0.05);microRNA-7与患者年龄无明显相关性(P>0.05)。结论 microRNA-7在胰腺癌患者组织中呈现低表达,且与临床分期和淋巴结转移与否具有一定相关性,可用于辅助诊断胰腺癌,亦可作为其临床病理特征分类的参考因素。
Objective To detect the expression of microRNA-7 in pancreatic carcinoma and analyze its clinical value in the diagnosis of pancreatic cancer. Methods To investigate the difference of expression level in pancreatic cancer tissue and benign diseases and to analyze its relationship with clinicopathological features of pancreatic cancer, and the expression of microRNA-7 was detected by fluorescence quantitative PCR in 53 cases of pancreatic cancer and adjacent tissues and 75 cases of benign pancreatic lesions. Results The expression of microRNA-7 gene in pancreatic cancer tissue was significantly lower than those in adjacent tissues and benign disease group, with the differences statistically significant(P<0.01);the concentration of microRNA-7 in III +IV patients were significantly lower than that of I+II(P<0.01);the concentration of microRNA-7 in lymph node metastasis patients were lower than those without metastasis significantly, with the differences statistically significant(P<0.05);microrna-7 had no significant correlation with patients’ age(P>0.05). Conclusion MicroRNA-7 showed lower expression in tissues of pancreatic cancer patients and was related to clinical staging and lymph node metastasis. It can be used to assist the diagnosis of pancreatic cancer.
作者
刘晓云
LIU Xiao-yun(Clinical Laboratory,Shengzhou People's Hospital(Shengzhou Branch of the First Hospital of Zhejiang University),Shengzhou,Zhejiang 312400,China)
出处
《中国卫生检验杂志》
CAS
2019年第4期466-468,共3页
Chinese Journal of Health Laboratory Technology
