摘要
目的探讨硫酸寡糖复合物(PI-88)对结肠癌SW620细胞迁移与侵袭的影响及机制。方法培养人结肠癌SW620细胞,分为对照组、溶媒组和药物组;对照组细胞不做任何处理,药物组细胞给予5μL的0.5mol/L的PI-88,溶媒组给予细胞5μL的磷酸盐缓冲液(PBS)。各组细胞处理后继续培养24h,采用划痕实验检测结肠癌SW620细胞迁移距离;Transwell小室检测结肠癌SW620细胞迁移数量与侵袭数量;Westernblot检测结肠癌SW620细胞中乙酰肝素酶(HPA)、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)蛋白的表达。结果划痕实验结果显示,药物组SW620细胞迁移距离(35.49±6.89)μm小于对照组(216.75±21.43)μm、溶媒组(227.52±17.72)μm,差异均有统计学意义(P值均<0.05)。Transwell小室细胞迁移、侵袭实验结果显示,药物组SW620细胞迁移数目(226±37)个和侵袭数目(89±11)个均少于对照组(875±93、321±28)个和溶媒组(843±116、331±43)个,差异均有统计学意义(P值均<0.05)。Westernblot实验结果显示,药物组PHA、VEGF、bFGF蛋白相对表达量均明显低于对照组和溶媒组,差异均有统计学意义(P值均<0.05)。结论PI-88具有抑制结肠癌SW620细胞迁移与侵袭的作用,其作用机制可能与PI-88抑制结肠癌SW620细胞HPA、VEGF、bFGF蛋白的表达有关。
Objective To explore the effects and the mechanism of sulfated oligosaccharide phosphomannopentaose sulfate (PI-88) on invasion and migration of colon cancer SW620 cells. Methods Colon cancer SW620 cells were cultured and further divided into control group, solvent group and drug group. The cells in the control group were treated without any treatment, the cells in the drug group were treated with 0.5 mol/L PI-88 5μL, and the cells in the solvent group were treated with 5 μL PBS of equal volume. The cells of each group were cultured for 24 h after treatment. The migration distance of colon cancer SW620 cells were analyzed by wound healing assay. The number of migration and invasion of colon cancer SW620 cells were analyzed by Transwell assay. Finally, the protein expressions of heparanase(HPA), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF) in colon cancer SW620 cells were detected by Western blot assay. Results The data of wound healing assay showed that the migration distance of SW620 cells in the drug group(35.49±6.89)μm was less than that in the control group(216.75±21.43)μm and the solvent group(227.52±17.72)μm, and the difference was statistically significant(all P values <0.05). The data of Transwell assay showed that the number of migration(226±37) and invasion(89±11) of colon cancer SW620 cells in the drug group were less than those in the control group(875±93, 321±28) and the solvent group(843±116, 331±43), and the difference was statistically significant(all P values<0.05). Moreover, the data of Western blot showed that the protein expressions of HPA, VEGF and bFGF in the drug group were decreased than those in the control group and the solvent group, and the differences were statistically significant(all P values<0.05). Conclusions PI-88 inhibits colon cancer cell migration and invasion, the mechanism may be related to the inhibition of HPA, VEGF and bFGF protein expressions.
作者
张磊
吴慧丽
肖兴国
李琨琨
张利
张超
Zhang Lei;Wu Huili;Xiao Xingguo;Li Kunkun;Zhang Li;Zhang Chao(Department of Gastroenterology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, China)
出处
《中华解剖与临床杂志》
2018年第6期543-547,共5页
Chinese Journal of Anatomy and Clinics
基金
河南省科技攻关计划项目 (162102310228).
关键词
结肠肿瘤
硫酸寡糖复合物
侵袭
迁移
SW620细胞
Colonic neoplasms
Sulfated oligosaccharide phosphomannopentaose sulfate
Invasion
Migration
SW620 cells
