摘要
目的采用胸膜穿刺针联合OB吻合胶构建615小鼠皮下移植瘤模型。方法小鼠前胃癌细胞株(MFC)在无菌环境下培养制成浓度1×10~7个/mL细胞悬液,然后将悬液接种于小鼠右腋下,建成胃癌动物皮下移植瘤模型,利用瘤块进行体内传代3次,胸膜穿刺针法进行穿刺移植建立615小鼠皮下移植瘤模型。同时建立对照组(未放瘤块)用于对比,小鼠成瘤情况利用外观形态学和HE染色方法评价。结果模型组小鼠体质量减轻。模型组肿瘤在第12天后生长较快,模型成功率为100%。外观形态学观察,模型组10只小鼠成瘤率100%,平均瘤重为(7.7160±0.2937)g;对照组成瘤率为0,模型组与对照组差异明显(P<0.01)。病理结果显示:模型组的瘤块体积较大,表面不规则,细胞核呈圆形、卵形或异形,核仁不显现,核分裂相较明显,是比较典型的胃癌组织的形态特征。结论胸膜穿刺针联合OB吻合胶法构建615小鼠胃癌皮下移植瘤模型成功率高、并且具有高效、便利等特点,值得推广应用及研究。
Objective A subcutaneous xenograft model of 615 mice was established by using pleural puncture needle and OB anastomosis glue. Method Mouse Forestomach Carcinoma Cell(MFC) was cultured under aseptic conditions to obtain a cell suspension with a concentration of 1×10^7 cells/mL. The suspension was inoculated into the right armpit of the mouse to establish a model of subcutaneous xenograft tumor of gastric cancer. The 615 mice subcutaneous xenograft model was built through puncture and transplation pleural needle method and by blocking three times in vivo passage. At the same time, a control group(without the tumor block) was used. The tumorigenesis of the mice was evaluated based on the appearance morphology and HE staining. Result Model mice lost weight. The model group grew faster since the 12 th day and the model success rate was 100%. In terms of appearance morphology, tumor formation rate of 10 mice in the model group was 100%;The average tumor weight(7.7160 ± 0.2937)g;The tumor rate of the control group was 0, thus the difference between the model group and the control group was significant(P<0.01).Pathological result show the typical morphological characteristics of gastric cancer tissue in that the nodules in the model group were larger in size, irregular in surface, round in nucleus, ovate or irregularly shaped. Besides,nucleolus did not appear and mitotic cells were more obvious. Conclusion The 615 mice gastric cancer subcutaneous xenograft model, built with pleural puncture needle combined with OB anastomosis gel is characterized by high success rate, high efficiency and convenience, thus it is worth further research and promotion.
作者
程诚
曾永蕾
王茎
吴勇
冷玉玲
孙春
臧刘兵
尹亚男
刘青
CHENG Cheng;ZENG Yonglei;WANG Jing;WU Yong;LENG Yuling;SUN Chun;ZANG Liubing;YIN Yanan;LIU Qing(Anhui University of traditional Chtnese medicine,Hefei 230001,China;The Second Affiliated Hospital of Anhui University of traditional Chinese medicine,Hefei 230061,China;School of traditional Chinese medicine,Anhui University of traditional Chinese medicine,Hefei 230038,China)
出处
《实验动物科学》
2018年第6期47-51,共5页
Laboratory Animal Science
基金
安徽省自然基金面上资助项目(No.1608085MH233)
安徽省高校科研创新平台建设项目(No.2015TD033)
关键词
胸膜穿刺针
小鼠前胃癌细胞株
OB吻合胶
615小鼠
Pleural puncture needle
Mouse Forestomach Carcinoma Cell
OB anastomosis glue
The 615 mice
