摘要
目的探讨婴儿巨细胞病毒肝炎不同证型的尿液代谢组学特征。方法筛选84例婴儿巨细胞病毒肝炎患儿分为湿热内蕴证(39例)、脾虚湿困证(28例)、气滞血瘀证(17例),另选健康对照组39名。采用气相色谱及质谱联用(GC-MS)技术对尿液样本进行代谢组学检测,经主成分分析寻找各证型的差异性代谢物。结果生化指标分析显示:湿热内蕴证与脾虚湿困证的总胆红素、结合胆红素及碱性磷酸酶水平存在差异(P <0. 05);湿热内蕴证与气滞血瘀证的天门冬氨酸氨基转移酶、总胆红素、结合胆红素、总胆汁酸水平及凝血酶原时间存在差异(P <0. 05);脾虚湿困证与气滞血瘀证的天门冬氨酸氨基转移酶、总胆红素、结合胆红素、总胆汁酸、碱性磷酸酶水平及凝血酶原时间存在差异(P <0. 05)。代谢组学研究显示:各证型在OPLS-DA图上区分明显,各证型间差异性代谢物涉及多种氨基酸代谢、能量代谢及肠道菌群相关代谢物紊乱。结论婴儿巨细胞病毒肝炎湿热内蕴证、脾虚湿困证及气滞血瘀证存在各自的尿液代谢组学特征。
Objective To investigate the urinary metabolomic characteristics of different Chinese medicine( CM)syndromes in infantile human cytomegalovirus hepatitis. Methods Totally 84 infantile human cytomegalovirus hepatitis patients were screened and assigned to damp-heat syndrome( 39 cases),Pi-deficiency with dampness encumbrance syndrome( 28 cases),Qi stagnation and blood stasis syndrome( 17 cases). Another 39 cases of normal control were collected at the same time. A non-targeted gas chromatography-mass spectrometry( GC-MS) metabolomics method was used in conjunction with orthogonal partial least squares discriminant analysis( OPLS-DA) to explore the differential metabolites and pathways of the three CM syndromes. Results In clinical index analysis,damp-heat syndrome and Pi-deficiency with dampness encumbrance syndrome differ in total bilirubin( TBIL),direct bilirubin( DBIL) and alkaline phosphatase( ALP,P < 0. 05).Damp-heat syndrome and Qi stagnation and blood stasis syndrome differ in aspartate aminotransferase( AST),TBIL,DBIL,total bile acid( TBA) and prothrombin time( PT,P < 0. 05). Pi-deficiency with dampness encumbrance syndrome and Qi stagnation and blood stasis syndrome differ in AST,TBIL,DBIL,TBA,ALP and PT(P < 0. 05). In metabolomic study,the OPLS-DA score plots revealed clear differentiation among the three CM syndromes. The differential metabolites between the three CM syndromes involve a variety of amino acid metabolism,energy metabolism and intestinal bacterial metabolism disorders. Conclusion Three CM syndromes of infantile human cytomegalovirus hepatitis have specific urinary metabolomic profiles,which indicated that different CM syndromes have their specific biological basis.
作者
李维薇
贺丽丽
单进军
谢彤
杜丽娜
杨燕
汪受传
LI Wei-wei;HE Li-li;SHAN Jin-jun;XIE Tong;DU Li-na;YANG Yan;WANG Shou-chuan(Department of Pediatric,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing(210023 );Institute of Pediatrics,Traditional Chinese Medicine,Nanjing University of Chinese Medicine,Nanjing(210023 );Department of Pediatric,Jiangsu Province Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing (210028);Department of Traditional Chinese Medicine,Beijing Children's Hospital Affiliated to Capita/Medical University,Beijing (100045))
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第2期162-168,共7页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81473725)
江苏省研究生科研与实践创新计划项目(No.KYCX18_1550)
关键词
婴儿巨细胞病毒肝炎
中医证候
代谢组学
湿热内蕴证
脾虚湿困证
气滞血瘀证
infantile human cytomegalovirus hepatitis
Chinese medicine syndromes
metabolomics
damp-heat syndrome
Pi-deficiency with dampness encumbrance syndrome
Qistagnation and blood stasis syndrome
