摘要
本文以氨氧化细菌(ammonia-oxidizing bacteria,AOB)特征基因氨单加氧酶(ammonia monooxygenase,AMO)α亚基基因(amoA)为目标基因(～491bp),分别采用克隆文库、454高通量测序和illumina测序技术对其群落结构进行了比较研究。结果表明, 454高通量测序和illumina测序技术得到的菌群多样性及丰富度均高于克隆文库技术。在菌群组成方面,虽然3种测序技术检测到的优势类群均为亚硝化螺菌,但在菌群丰度方面存在差异。3种测序技术中,克隆文库技术在很大程度上可能高估物种丰度,且对部分低丰度类群的检测能力有限;illumina单端测序由于其测序片段较短,可能存在物种注释错误、不能准确区分各物种间序列差异等问题;比较而言, 454高通量测序技术能较为全面和准确地反映海洋沉积物中AOB的菌群结构特征。
In this study, we evaluated the advantages and disadvantages of clone library, pyrosequencing, and illumina sequencing of ammonia-oxidizing bacteria (AOB) communities in marine sediments using ammonia monooxygenase α subunit (amoA) (-491 bp). Results showed that the diversity and richness of AOB communities based on pyrosequencing and illumina sequencing were much higher than those obtained by traditional clone library. Although Nitrosospira was the predominant species detected by the three sequencing technologies, there were still some differences in its abundance. Among these three sequencing analyses, clone library may overestimate the abundance of species, and it is also limited for some low-abundance groups. Due to its short fragments, single-end illumina sequencing may result in annotation errors and cannot accurately distinguish divergence among sequences. Thus, pyrosequencing could provide a relatively more accurate and comprehensive description of AOB community structure in marine sediments.
作者
贺惠
陈阳阳
王勋功
甄毓
米铁柱
于志刚
李迎
HE Hui;CHEN Yang-yang;WANG Xun-gong;ZHEN Yu;MI Tie-zhu;YU Zhi-gang;LI Ying(College of Marine Life Science,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,China;Key Laboratory of Marine Environment and Ecology,Ministry of Education, Qingdao 266100,China;College of Environmental Science and Engineering,Qingdao 266100,China;Key Laboratory of Marine Chemical Theory and Technology,Ministry of Education,Qingdao 266100, China)
出处
《海洋科学》
CAS
CSCD
北大核心
2018年第8期22-29,共8页
Marine Sciences
基金
国家自然科学基金项目(41521064
41620104001)
中国科学院海洋生态与环境科学重点实验室
青岛海洋科学与技术国家实验室海洋生态与环境科学开放课题(KLMEES201601)~~
