摘要
为提高白芽奇兰茶的利用率,利用蜗牛酶辅助提取白芽奇兰茶多糖,在单因素试验基础上,以多糖提取率为响应值,采用Box-Behnken方法对酶解温度、酶用量、酶解时间和pH进行四因素三水平试验设计,利用Design-Expert 8.05b软件对白芽奇兰茶多糖的酶解提取工艺进行响应面优化,利用白芽奇兰茶多糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟基(OH)自由基和超氧阴离子(O2-)自由基的清除能力来评价其抗氧化活性。结果表明,最佳的提取工艺条件为酶解温度51℃、酶用量1.2%、酶解时间55 min和pH 5.6,得到白芽奇兰茶多糖的提取率为93.81mg·g^(-1),与未加酶相比,提取率提高了77.74%。试验显示,白芽奇兰茶多糖与DPPH自由基、OH自由基和O_2^-自由基的清除效果有明显的量效关系,对DPPH自由基、OH自由基和O_2^-自由基的IC50分别为80.65,204.39和428.10 mg·L^(-1)。
A snail enzyme-assisted extraction of polysaccharides from Baiyaqilan tea was used to improve the utilization ratio of Baiyaqilan tea. Based on the single-factor experiment, a four-factor and three-level experiment was designed by the Box-Behnken method using extraction yield of polysaccharides as response value, enzymolysis temperature, enzyme dosage, enzymolysis time and pH as factors. The enzymatic extraction technology of polysaccharide from Baiyaqilan tea was optimized using response surface methodology by Design-Expert 8.05 b software. The scavenging capacity of DPPH, OH and O^2- free radicals was used to evaluate its antioxidant activity. The results showed that the optimum extraction parameters were as follows: temperature 51℃, enzyme dosage 1.2%, enzymolysis time 55 min and pH 5.6, and the extraction yield was up to 93.81 mg·g^-1. The extraction rate of this process increased 77.74 % than that of the hot water extraction without snail enzyme. Experiment results showed that there was a significant dose-effect relationship between the scavenging effects of polysaccharides from DPPH, OH and O^2- free radicals, the IC50 values of DPPH, OH and O^2- free radicals were 80.65, 204.39 and 428.10 mg·L^-1, respectively.
作者
陈文娟
陈建福
卢惠婷
庄远红
CHEN Wenjuan;CHEN Jianfu;LU Huiting;ZHUANG Yuanhong(Department of Food Engineering,Zhangzhou City University,Zhangzhou 363000;School of Food Engineering,Zhangzhou Institute of Technology,Zhangzhou 363000;Collaborative Innovation Center of Fine Chemicals in Fujian Province,Zhangzhou 363000;Department of Biological Science and Technology,Minnan Normal University,Zhangzhou 363000)
出处
《安徽农业大学学报》
CAS
CSCD
2018年第6期996-1003,共8页
Journal of Anhui Agricultural University
基金
漳州市自然科学基金(ZZ2017J43)
福建省高校杰出青年科研人才培育计划(闽教科[2015]54号
福建省中青年教师教育科研项目(JAT171090)共同资助
关键词
白芽奇兰
茶多糖
蜗牛酶
抗氧化
Baiyaqilan tea
tea polysaccharides
snail enzyme
antioxidant activity
