摘要
目的 :在豚鼠哮喘模型上观察心、肝、肾、肺等组织金属硫蛋白 (metallothionein ,MT)含量的变化 ,探讨其内源性防御机制。方法 :以卵蛋白诱发豚鼠哮喘 ,分别以 [10 9Cd]血红蛋白饱和法和硫代巴比妥酸法测定心、肝、肾、肺组织及血浆MT和丙二醛 (MDA)的含量。结果 :豚鼠哮喘发病时 ,心、肝、肾、肺和血浆MT含量分别比对照组高 2 2 %、170 %、6 6 %、78%、30 % (均P <0 0 1) ,心、肝、肾、肺组织及血浆MDA的含量分别比对照组高 10 9 30 %、6 2 6 6 %、71 4 2 %、72 0 1%、5 2 30 %(均P <0 0 1)。结论 :哮喘豚鼠体内MT生成增多 ,可能是机体的重要防御保护性反应 ,其病理生理意义值得进一步探讨。
Objective: To search for the mechanism of organism endodefence, the changes of metallothionein (MT) in heart, liver, kidney, lung and plasma of guinea pig with asthma were examined Methods: The contents of MT in heart, liver, kidney, lung and plasma of the guinea pig with asthma induced by egg protein administration were radiometrically and chemically measured. Results:Compared with control, the concentration of MT and MDA in the tissues of heart, liver, kidney, lung and plasma significantly increased by 22%, 170%, 66%,78%,30% respectively(P<0 01) The MDA levels in heart, liver, kidney, lung and plasma in asthma group increased by 109.30%,62.66%,71.42%,72.01%,52.30% respectively (P<0 01).Conclusion: The MT was generated more in asthma guinea pig, it may be an important reaction of protection and defense. The pathophysiological significance of the present finding deserves to be further explored.
出处
《宁夏医学院学报》
2002年第1期4-6,T001,共4页
Journal of Ningxia Medical College
基金
20 0 1年宁夏教委资助课题 (课题号码 :12 2 )
