摘要
目的 观察抗癌新药 2 0 (R) 人参皂甙Rg3(2 0 (R) ginsenoside ,Rg3)对K5 6 2 /ADM多耐药细胞株逆转MDR机制。方法 应用MTT法确定ADM和Rg3的细胞毒 ;用荧光分光光度仪测定K5 6 2 /ADM细胞内阿霉素 (Adriamycin ,ADM )的浓度 ;流式细胞仪检测细胞凋亡率和表达P 170糖蛋白 (P glycoprotein ,Pgp)的K5 6 2 /ADM的细胞含量。 结果 (1)K5 6 2 /ADM细胞对阿霉素 (Adriamycin ,ADM )的抗性比K5 6 2细胞高 11倍 ,但两者对Rg3的IC50 分别为 11 76± 0 33μg/ml、10 4 9± 0 30 μg/ml,无显著性差异 (P >0 0 5 )。(2 )无毒剂量和低毒剂量的Rg3能显著提高K5 6 2 /ADM细胞内ADM的浓度 ,使该细胞对ADM的敏感性明显提高。 (3)Rg3对K5 6 2 /ADM细胞有较强的抑制生长和诱导凋亡作用 ,并有时间和浓度依赖性。(4)Rg3对表达P 170糖蛋白的K5 6 2 /ADM细胞的百分含量无显著性影响。结论 2 0 (R) 人参皂甙Rg3不仅是抑制肿瘤生长和转移的抗瘤剂 。
Objective To observe and study the mechanism of reversing multidrug resistance (MDR) of K562/ADM cell line by a new anti plasma drug 20(R) ginsenoside Rg3. Methods MTT was applied to determine the cytotoxicity of ADM and Rg3 to K562/ADM cell; The concentration of ADM in K562/ADM cell was detected by fluorescence spectrophotometer; The apoptosis rate and quantity of K562/ADM expressing P glycoprotein(Pgp) were tested by flow cytometry (FCM); Transmission electron microscope (TEM) was used to observe apoptotic K562/ADM cells and apoptotic bodies. Results (1) The resistance of K562/ADM cell to ADM was 11 times as high as K562 cell was. IC 50 value of Rg3 on K562 and K562/ADM cell were 10.49±0.30μg/ml and 11.76±0.33μg/ml respectively. There wasnt obviously different (P>0 05). (2) The concentration of ADM in K562/ADM cells can be lifted by innocurity and low toxicity dose of Rg3 obviously, the sensitivity of K562/ADM cell to ADM was raised remarkably. (3) Rg3 can quite inhibit growth of K562/ADM cells and induce apoptosis. These actions had dependence on acting time and concentration of Rg3. (4) The percent of K562/ADM cells of expressing Pgp wasnt affected by Rg3. Conclusion 20(R) ginsenoside Rg3 was not only an anti plasma agent but also a reversal agent of MDR and inducer of apoptosis.
出处
《解剖科学进展》
CAS
2002年第1期31-35,共5页
Progress of Anatomical Sciences
