摘要
用一种由 1 .5 % SDS,1 0 0 m mol/ L Tris( p H8.3) ,5 0 m mol/ L EDTA( p H8.0 ) ,5 0 0 m mol/L Na Cl和 5 0 0 m mol/ Lβ-巯基乙醇组成的 SDS提取液保存野外采集的刺桫椤 ( Alsophila spinulosa)叶片组织 ,结果表明 ,保存 7d、 1 5 d、 30 d、 90 d、 1 80 d(室温下 )的刺桫椤叶片组织提得的 DNA分子量与鲜叶一致 ,均大于 4 8kb,得率为 6 0 0～ 1 2 0 0 μg/ g· FW.采自同一植株不同保存时间的叶片组织获得的 DNA用于 RAPD分析 ,结果完全一致 .用该方法保存的 8个刺桫椤天然居群的 91份样品 ,提得的
A preservative medium which consisted of 1 5 % SDS (sodium dodecyl sulfate), 100 m mol/L Tris (pH 8 3), 50 m mol/L EDTA (pH 8 0), 500 m mol/L NaCl and 500 m mol/L β sulfhydryl ethanol, was applied to field preservation of leaves tissue for DNA preparation of Alsophila spinulosa . It was indicated that the isolated DNA from the leaves tissue preserved in the medium for 7d?15d?30d?90d?180d was 48 kb, and DNA yield was 600～1200 μg/g·FW. This results was highly similar to that from fresh leaves tissue. The isolated DNA from the preserved leaves tissue were used for RAPD analyzing and it was showed that the preserved leaves tissue from same individual was obtained a same result as from fresh leaves. High quality DNA of 91 individuals from 8 populations of Alsophila spinulosa was prepared by this method. This isolated DNA has been use to experiments base on PCR technology.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
2002年第1期82-85,共4页
Journal of Fujian Normal University：Natural Science Edition
基金
福建省教育委员会科学基金项目 ( JA9914 3)
