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使用含多肽因子牙膏后牙釉质钙含量的电子探针分析 被引量:1

Determination of Ca^(2+) Content in Human Enamel by Electroprobe After Polypeptide Tooth Paste Usage
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摘要 目的 观察含生物素多肽因子牙膏对牙釉质再矿化的作用。方法 在体外pH循环环境中 ,选择釉质完整的 4颗离体牙做成 8枚釉质片 ,分成实验和对照 2组 ,分别用含多肽因子的牙膏和不含多肽因子牙膏进行处理 ,然后在第 12d和第 15d取样 ,进行电子探针检测牙釉质中的钙含量。通过检测图像分析含多肽因子牙膏对牙釉质的再矿化作用。结果 第 12d和 15d电子探针检测图像显示 :实验组牙釉质中总的钙含量高于相应的对照组。分析发现 ,随着实验时间的增加 ,实验 15d组釉质片的钙含量高于 12d组。同时 ,对照组釉质深层有较多的钙溶出 ,并在其表面重新沉积 ,而实验组的深层釉质钙溶出较少。结论 含多肽因子牙膏有助于阻滞溶解的钙离子逸出 。 Objective The purpose of this study was to investigate the capability of polypeptide tooth paste for remineralization of human enamel.Methods 8 enamel slabs, which were taken from extracted tooth for orthodontics reason, were immersed in artificial cariogenic solution for 0.5h. Then, the slabs were treated by tooth paste with polypeptide and tooth paste without polypeptide for 5 min. After tooth paste were washed away by warm water, the slabs were immersed in artificial saliva. The experiment were carried out twice per day for 15 days. Two slabs were taken from test group (treated by tooth paste with polypeptide) and control group (treated by tooth paste without polypeptide) on the twelfth day and fifteenth day, respectively. The Ca 2+ content in enamel slabs were determined by electroprobe and analyzed by image process.Results The content of Ca 2+ in test group was higher than that in control group significantly.Conclusion The polypeptide tooth paste has certain capability of inhibiting demineralization for human enamel.
出处 《上海口腔医学》 CAS CSCD 2002年第1期46-47,共2页 Shanghai Journal of Stomatology
关键词 多肽因子牙膏 牙釉质 电子探针 钙含量 Enamel Polypeptide Electroprobe
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