摘要
目的 探讨小剂量化疗药物〔高三尖杉酯碱 (HHar)、阿克拉霉素 (ACR)、阿糖胞苷 (Ara- c)〕治疗急性白血病 (AL )的可能机制。方法 以成人 AL原代细胞为研究对象 ,充分利用其遗传性和体内细胞相似的特点 ,模拟人体用药后的最大血药浓度 ,对临床常用的三种小剂量化疗药物进行研究。首先采用光镜观察细胞超微结构变化 ,DNA凝胶电泳、二苯胺法、流式细胞术等一系列国内外检测细胞凋亡的经典方法 ,充分证实它们确可诱导 AL 原代细胞凋亡 ;尔后 ,进一步选取其中较为经济的光镜计数细胞凋亡率、二苯胺法定量测定凋亡细胞的DNA片段化率及台盼蓝染色光镜下计数坏死率三种方法来观察药物对急性淋巴细胞白血病 (AL L)及急性髓细胞白血病 (AML)原代细胞的诱导凋亡和杀伤 ,得出相应的时间—效应关系 ,并进行对比分析。结果 三种药物均可诱导 AL 原代细胞凋亡 ,对比分析显示 ACR的作用最强 ;AML 原代细胞对 HHar诱导凋亡的敏感性高于 AL L原代细胞。结论 诱导 AL 细胞凋亡可能是小剂量化疗药物治疗 AL 的主要机制 ;AL L 白血病细胞对 HHar诱导凋亡的作用不敏感可能是临床上 HHar对 AL L
Objective To explore the possible mechanism of low-dose chemotherapeutic drugs in treating acute leukemia.Methods Primary acute leukemia cells of 34 patients (AML 19,ALL 15)were treated with 3 chemotherapeutic drugs:homoharringtonine(HHar),aclarubicin A(ACR) and arabinoside(Ara-c).Light microscopy,DNA gel electrophoresis diphenylamine reaction and cytometry were used to detect cell apoptosis,and trypan blue stain to detect cell necrosis.Finally,the time-effect relationships were analyzed furthermore.Results While all of the three drugs can induce the apoptosis of primary acute leukemia cells ACR is the most effective one.The AML primary cells were more sensitive to the apoptosis induced by HHar than the ALL ones.Conclusion Inducing apoptosis may be the predominant mechanism of low-dose chemotherapeutic drugs in treating acute leukemia.The AML primary cells were more sensitive to the apoptosis induced by HHar than the ALL ones.And these may be the possible mechanism that HHar is more effective in AML than in ALL.
出处
《山西医药杂志》
CAS
2002年第2期102-104,共3页
Shanxi Medical Journal
