期刊文献+

动脉内膜损伤后平滑肌细胞增殖与凋亡及相关基因表达 被引量:2

Cell apoptosis, proliferation and related-gene expression in smooth muscle after arterial intimal injury
下载PDF
导出
摘要 目的 :探讨动脉内膜损伤后狭窄的发生机制。方法 :用 2 4只新西兰大白兔建立腹主动脉下端内膜损伤后狭窄模型 ,采用原位末端标记法 (TUNEL)、免疫组化和原位杂交方法 ,检测增殖内膜中血管平滑肌 (VSMCs)增殖、凋亡及相关基因c fos、c myc、p5 3和PCNA的表达。 结果 :术后 1~ 2周PCNA达到高峰 ,与 4周~ 12周组间差异有显著性 (P <0 0 5 ) ,TUNEL在术后4个月内保持低水平 ,损伤后 4个月内VSMCs凋亡低于增殖水平。术后 1~ 2周 ,c fos、c mycmRNA表达强阳性 ,在 3个月后未见表达。p5 3只在 1周内有弱阳性而其余各时间点未见表达。 结论 :VSMCs增殖和凋亡的失衡与动脉内膜损伤后狭窄密切相关 ,c fos和c myc参与了VSMCs凋亡和增殖的调节 。 purpose To study the mechanism of arterial intimal stenosis. Methods Stenosis models of ventral aorta after arterial intimal injur were established in 24 rabbits. TUNEL and immunohistochemistry and in situ hybridization were used to detect the expression of apoptosis and proliferation related gene c fos, c myc and p53 and proliferation cell nuclear antigen (PCNA) of smooth muscle cell (SMC) in injuryed intimae. Results From 1 to 2 weeks, the expression of PCNA reached its peak value that was different from the group of 4 to 12 weeks ( P <0 05). The level of TUNEL was low in the period of 1 week to 4 months. Intimal proliferation was accompanied with lower rate of apoptosis. The results of in situ hybridization showed that SMCs of neointima in the 1~2 weeks group displayed intense c myc, c fos mRNA staining,and no positivity after 3 months. But p53 staining was slightly positive only in the first week. Conclusions The imbalance of apoptosis and proliferation may be related to the restenosis after arterial intimal injury. c myc and c fos may involve in the regulate of apoptosis and proliferation of VSMCs. Adopting the mixed strategy to regulation the balance between apoptosis and proliferation may be useful to prevent arterial intimal stenosis.
出处 《临床与实验病理学杂志》 CAS CSCD 2002年第1期76-78,共3页 Chinese Journal of Clinical and Experimental Pathology
基金 江苏省教育厅自然科学基金 (No 98KJB3 10 0 0 2 )
关键词 动脉内膜损伤 血管损伤 平滑肌 基因表达 细胞增殖 细胞凋亡 arteries tanica intima injuries muscle,smooth,vascular proliferation apoptosis gene expression rabbits
  • 相关文献

参考文献1

二级参考文献2

共引文献4

同被引文献15

  • 1刘宁波,彭韬,沈波,冷静.选择性抑制剂celecoxib诱导肝癌细胞凋亡及其相关分子机制的实验研究[J].南京医科大学学报(自然科学版),2004,24(4):321-324. 被引量:11
  • 2邱雪杉,李宗铉,谢成耀.实验性动脉内膜增厚过程中平滑肌增殖的动态研究[J].解剖科学进展,1997,3(4):353-356. 被引量:1
  • 3Korotkova M,Westman M,Gheorghe KR,et al.Effects of antirheumatic treatments on the prostaglandin E (2) biosynthetic pathway[J].Arthritis Rheum,2005,52:3439-3447
  • 4Linto MF,Fazio S.Cyclooxygenases and inflammation in atherosclerosis[J].Curr Opin Pharmacal,2004,4:116-123
  • 5Williams CS,Mann M,DuBois RN.The role of cyclooxygenases in inflammation,cancer,and development[J].Oncogene,1999,18:7908-7916
  • 6Casterella PJ,Teirstein PS.Prevention of coronary restenosis[J].Cardiol Rev,1999,7:219-231
  • 7Tamburino c,Ussia GP,Zimarino M,et al.Early restenosis after drug-eluting stent implantation:A putative role for platelet activation[J].Can J Cardiol.2007,23(1):57-59
  • 8Leng J,Hang C,Demetris AJ,et al.Cyclooxygenase-2 promotes hepatocellular carcinoma cell growth through Akt activation:ebidence for Akt inhibition in celecoxib induced apoptosis[J].Hepatology,2003,38:756-768
  • 9Wu T,Leng J,Han C,et al.The cyclooxygenase-2 inhibitor celecoxib blocks phosphorylation of Akt and induces apoptosis in human cholangiocarcinoma ceHs[J].Mol Cancer Ther,2004,3:299-307
  • 10Zhang GS,Liu DS,Dai CW,et al.Antitumor effects of celecoxib on K562 leukemia cells are mediated by cellcycle arrest,caspase-3 activation,and downregulation of Cox-2 expression and are synergistic with hydmxyurea or imatinib[J].Am J Hematol,2006,81(4):242-255

引证文献2

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部