摘要
目的:研究慢性乙型肝炎患者血清HBVDNA含量对IL-12诱导其PBMC产生Th1/Th2类细胞因子协同效应的影响。方法:分离 50例慢性乙型肝炎患者外周血单个核细胞,分别与 PHA(100 ug/ml)、HBcAg(1ug/ml)、HBeAg( 1 ug/ml)单独或联合IL-12(10 ng/ml)体外培养48 h,ELISA法检测培养上清液细胞因子 IL-2、IFN-γ、IL-4、IL-10。荧光定量PCR检测患者血清 DNA含量,并分成 HBVDNA小于103拷贝/ml、103~105拷贝/ml、105~107拷贝/ml、大于 107拷贝/ml4组。结果:以 HBVDNA小于103拷贝/ml组做对照组,比较发现无论抗原(PHA、HBcAg、HBeAg)单独诱导还是联合IL-12共同诱导,随着血清HBVDNA含量的增高,PBMC产生IL-2和IFN-γ水平逐渐降低,产生IL-4和IL-10水平逐渐升高,并且IL-12对PBMC产生IFN-γ的增殖效应逐渐减弱,特别是血清HBVDNA大于 107拷贝/ml患者几乎无明显增殖效应。结论:高水平血清HBVDNA含量对IL-12诱导慢性乙型肝炎PBMC产生IFN-γ协同效?
Objective:To examine the influence of the HBV virus load on co-stimulatory effect of rhIL-12 on Th1/Th2-type cytokines production of PBMC in chronic hepatitis B virus infection patients. Methods: PBMC of 50 chronic hepatitis B virus infection patients were stimulated with PHA(100 U/ml),XHBcAg(1 ug/ml),HBeAg(1 ug/ml) in the presence or obsence of IL-12(10ng/m1).IL-2,JFN-y,LL-4,IL-10 production were determined by EUSA after 48 hours. HBVDNA in serum were determined by fluorecence quantity PCR(FQ-PCR) . The patients were divided into 4 groups(HBVDNA< 103 copies/ml, 103 - 105 copies/ml, 105 - 107 copies/ml, >107 copies/ml). Results: After stimulation with IL-12 and HBcAg/HBeAg or PHA ,co-stimulatory effect on IFN-y production were decreased while level of HBVDNA in serum was high. Co-stimulatory effect on IFN-y production almost was not observed in HBVDNA> 107 copies/mi group. Conclusion: The presence of high amount of HBVDNA in serum is associated with suppressed co-stimulatory and regulatory effect of IL-12 to Th1/Th2 profilation
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2002年第4期276-278,共3页
Chinese Journal of Immunology