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简单快速筛选抑制黄曲霉生长的乳酸菌的方法 被引量：2

A simple method for screening of highly inhibitory Lactic acid bacteria on Aspergillus flavus growth
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摘要 使用夹层培养基法 ,将乳酸菌接种于MRS琼脂与PDA琼脂之间 ,在PDA表面涂布一定量的霉菌孢子 ,2 8℃培养 72小时后 ,计数霉菌菌落 ,即可初步判定乳酸菌是否对霉菌孢子有抑制作用。经多次重复试验 ,与目前常用方法相结合 。 A simple and rapid method for screening of Lactic acid bacteria with high inhibitory power is developed. The methodology employed was the agar-diffusion assay, which was standardized for medium composition, and incubation conditions. The assay was composed of two layers of medium, MRS agar at the bottom of plate, PDA at the top of MRS agar. Lactic acid bacteria culture was inoculated to the media between MRS and PDA, then a known number of mold spores was surface plated on PDA plates, and incubated at 25℃ for 72h.
作者 徐进 计融 杨宝兰 李志刚 罗雪云
机构地区 卫生部食品卫生监督检验所
出处 《卫生研究》 CAS CSCD 北大核心 2002年第2期122-123,共2页 Journal of Hygiene Research
关键词 乳酸菌 抑制 黄曲霉 筛选 Lactic acid bacteria, Aspergillus flavus, inhibition
分类号 R155.57 [医药卫生—营养与食品卫生学] TS252.54 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献5

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同被引文献20

  • 1励建荣,朱丹实.果蔬保鲜新技术研究进展[J].食品与生物技术学报,2012,31(4):337-347. 被引量：73
  • 2彭坚,韩北忠.小麦中黄曲霉生长的预测模型[J].河南工业大学学报（自然科学版）,2005,26(2):51-54. 被引量：15
  • 3Whiting R C, Buchanan R L. A classification of models for predictive mierobiology[J]. Food Microbiology,1993,10:175- 177.
  • 4Maren A Klich. Environmental and developmental factors influencing aflatoxin production by Aspergillus flavus and Aspergillus parasiticus[J]. Mycoscience,2007,48:71-80.
  • 5Robin C McKellar. Effect of sub-lethal heating and growth temperature on expression of the ribosomal RNA rrnB P2 promoter during the lag phase of Pseudomonas fluorescens [J]. International Journal of Food microbiology,2007,116 (2):248-259.
  • 6Ratkowsky D A, Olley J, McMeekin T A, et al. Relationship between temperature and growth rate of bacterial cultures[J]. Journal of Bacteriology, 1982,149:1-5.
  • 7Vasilopoulos C, Mev ED, Dewulf D, et al. Interactions between bacterial isolates from modified-atmosphere-packaged artisan- type cooked ham in view of the development of a bioprotective culture[J]. Food Microbiology, 2010,27 : 1086-1094.
  • 8Trias R, Badosa E, Montesinos E, et al. Bioprotective Leuconostoc strains against Listeria monocytogenes in fresh fruits and vegetables[J]. International Journal of Food Microbiology, 2008,127 : 91-98.
  • 9Corsetti A,Gobbetti M,Rossi J,et al. Antimould activity of sourdough lactic acid bacteria:Identification of a mixture of organic acids produced by Lactobacillus sanfrancisco CB1 [J]. Applied Microbiology and Biotechnology, 1998,50: 253-256.
  • 10Villarreal JV, Schillinger U. Inhibition of Penicillium nordicum in MRS medium by lactic acid bacteria isolate from foods[J]. Food Control, 2010,21 : 107-111.

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卫生研究
2002年 第2期

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