摘要
本研究旨在探讨宫颈癌前病变和宫颈癌的发生发展与人乳头瘤病毒及协同因子 (HSV ,CMV)的关系。对81例不同宫颈病变组织进行HPV16 / 18和HPV6 / 11原位杂交 ,同时对 10 3例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。结果表明病毒DNA原位杂交信号的分布与HE染色中挖空细胞的分布一致。HPV16 / 18与不同宫颈病变组织原位杂交阳性率平均为 5 1% ,HPV6 / 11的则为 6 4%。经PCR检测 ,HPV16 / 18、HPV6 / 11、HSV、CMV在不同宫颈病变组织中的阳性率分别为 2 1%、4%、2 3%和 0 %。HSV可协同HPV16 / 18恶性转化宫颈上皮细胞 。
The carcinogenesis of the human cervical precancerous lesion,cervical carcinoma is known closely associated with human papillomavirus (HPV).The purpose of this article is to identify whether HSV and CMV play as co-factor role in the carcinogenesis.Eighty-one cases of various cervical lesions were analyzed by HPV6/11,HPV16/18 in situ hybridization.Meanwhile,HPV,HSV and CMV were determined in 103 cases of various cervical lesions.The results show that the distribution of positive hybridization signal was consistent with the distribution of Koilocytic cells in HE stain.Of these cervical specimens investigated,the positive rates of HPV16/18 and HPV6/11 using ISH were 51% and 64%,respectively,the infection rates of HPV16/18,HPV6/11,HSV and CMV using PCR were 21%,4% 23% and 0%,respectively.The co-operation effect of HPV and HSV occurred in the oncogenesis of human cervical carcinoma,and moreover,the cellular and molecular biological mechanisms were discussed.
出处
《遗传》
CAS
CSCD
北大核心
2002年第2期121-124,共4页
Hereditas（Beijing)
基金
国家自然科学基金 ( 30 16 0 0 90 )
广东省医学科研基金 (A2 0 0 0 6 41)
中国科学院淡水生态与生物技术国家重点实验室开放课题基金资助项目 ( 1999FB0 10 )
关键词
宫颈癌
人乳头瘤病毒
原位杂交
协同因子
cervical carcinoma
human papillomavirus
in situ hybridization
