摘要
目的 考察四逆汤有效成分不同组合抗心肌缺血再灌注损伤的作用。方法 采用正交试验法 ,以超氧化物歧化酶 (SOD)、丙二醛 (MDA)以及乳酸为指标 ,考察附子生物碱 (A)、干姜挥发油 (B)、甘草酸粗品 (C) 3个因素。结果 3种有效成分最佳剂量为 A4B4C4,3因素对 SOD和 MDA影响大小顺序为 A>C>B,对乳酸影响大小顺序为 A>B>C。结论 3种有效成分临床极量组合的疗效最佳。附子生物碱是四逆汤有效成分组合中的关键因素 。
Object To study the protective effect of SINI TANG comprising active components combined at different dosage on myocardial ischemia reperfusion injury in mice. Methods In the studies, the experiment was performed by orthogonal design, the levels of SOD, MDA and lactate in cardiac muscle were regarded as criterion for evaluation of the every composition, three active components were studied including the alkaloid (A) from Aconitum carmichaeli Debx., the volatile oil (B) from Zingiber officinale Rosc. and crude glycyrrhizic acid (C) from Glycyrrhiza uralensis Fisch. Results The optimal composition comprised A at dose of 31.2 mg/kg, B at dose of 1.84 μL/kg and C at dose of 4.68 mg/kg (A 4B 4C 4). The effect of three active components on SOD and MDA was A>C>B and lactate was A>B>C. Conclusion The optimal SINI TANG composition comprises three active components combined at clinical maximal dosage showed the best therapeutic efficacy, of which A is a key factor and B, C are necessary factors for the composition in SINI TANG.
出处
《中草药》
CAS
CSCD
北大核心
2002年第4期333-336,共4页
Chinese Traditional and Herbal Drugs
基金
中山大学"2 11工程"重点建设项目 ( 98176 )
广东省中医药管理局科研基金项目 ( 10 0 118)
