摘要
目的 探讨细胞周期调控基因ｐ２７ｋｉｐｌ在膀胱移行细胞癌中表达及与临床病理特征之间的关系．方法 应用鼠抗人ｐ２７ｋｉｐｌ单克隆抗体对６５例膀胱移行细胞癌（ＢＴＣＣ）和１２例正常粘膜进行免疫组化ＳＰ法染色，观察ｐ２７ｋｉｐｌ的表达情况。结果ｐ２７ｋｉｐｌ在ＢＴＣＣ和正常膀胱粘膜中的阳性表达率分别为３２．３％和７５．０％，ＢＴＣＣ显著低于正常膀胱粘膜（Ｐ＜０．０１）；ｐ２７ｋｉｐｌ在ＢＴＣＣ中表达随着临床分期和病理分级的增高而降低（Ｐ＜０．０５），有淋巴结转移组显著低于无淋巳结转移组（Ｐ＜０．０５）。结论 ｐ２７ｋｉｐｌ对ＢＴＣＣ的分化、浸润和转移可能起抑制作用。检测ＢＴＣＣ中ｐ２７ｋｉｐｌ表达对ＢＴＣＣ的生物学行为评价和判断预后具有重要意义．
Objective To investigate the expression of cell cycle modulation gene p27kipl in bladder transitional cell carcinoma (BTCC )and evaluate its relationship with the pathological characteristic of BTCC. Methods With mouse anti-human p27kipl monoclonal antibody, immunohistochemistiy assay was used to examine the expression of p27kipl in 65 cases of BTCC and 12 cases of normal bladdeer tissue. Results The positivity rates of p27kipl in BTCC and normal bladder tissue were 32.3% and 75.0% respectively, and the former was significantly lower than the latter (P<0.01).There was a negativity correlation between p27kipl expression and the clinical stage, pathological grade of BTCC (P<0.05 ). The positivity rates of p27kipl (P<0.05 )in lymphoid node metastasized BTCC group were significantly lower than that in non-metastasized group (P<0.05). Conclusion p27kipl may play a role in inhibition of the differentiation .infiltration and metastasis of BTCC ,and the examination of p27kipl expression in BTCC may have an important theoretical significance to judge its prognosis and biological behavior.
出处
《实用医药杂志》
2002年第3期193-195,共3页
Practical Journal of Medicine & Pharmacy
