摘要
为了解航天诱变对菊苣不同品系保护酶活性的影响,为南方喀斯特地区筛选耐旱植物提供科学依据,以‘实践八号’育种卫星搭载选育出的菊苣新品系和目前主推的菊苣品种为研究对象,对SOD活性、POD活性、CAT活性以及丙二醛含量进行测定。结果表明:随着水分胁迫时间的增加,PA-82、普那菊苣和PA-43的丙二醛含量逐渐升高,在处理12 d时,分别上升了0.575、0.72和0.844μmol/g;SOD、POD和CAT活性迅速升高,在处理9 d时,PA-82、普那菊苣和PA-43的SOD分别上升了878.9、809.2和711.1 U/g,上升幅度为139.1%、136.7%和121.1%;POD分别上升了4397、3754和2767 U/(g·min),上升幅度为265.84%、257.65%和204.06%;CAT分别上升了402.1、277.1和170.9 KU/L,上升幅度为73.08%、39.69%和26.20%;之后3种保护酶均出现大幅度下降,12 d时,PA-82、普那菊苣和PA-43的SOD分别下降1206.5、1144.7和1108.6 U/g,POD分别下降3145、2876和2753 U/(g·min),CAT分别下降587.2、698.1和584.1 KU/L。经综合评价,3种菊苣的抗旱能力为PA-82>普那菊苣>PA-43。
In order to investigate the effect of space mutation of new strains of C. irtybus on the protective enzyme and provide the scientific theoretical basis for drought-tolerant plant screening in South China Karst region. The seeds of C. iraybus varieties were carded into outer space by ＇ Shijian No. 8＇ satellite after breeding of the new C. intybuJ L. strains and the primary varieties were used to study, meanwhile, activities of catalase （CAT）, peroxidase （ POD）, superexide dismutnse （SOD） and raalondialdehyde （MDA） content were determined. The resales showed that during the treatment of 12 d, the malondialdehyde （MDA） content of PA-82, Puna Chicory, PA-43, were increased by 0.575,0.72 and 0. 844 μmoL/g;activities of catalase （ CAT）, peroxidase （ POD ） and superoxide dismutase （ SOD ） were raised first, at 9 days, PA-82, Puna Chicory, PA-43 and SOD were increased by 878.9,809.2,711.1 U/g and the increase was 139.1%, 136.7 % and121.1%; POD were increased by 4397, 3754, 2767 U/（ g · min） and the increase was 265.84 %, 257.65 %, 204.06 %, CAT were increased by 402.1,277.1, 170.9 KU/L and the increase was 73.08 %, 39.69 %, 26.20 % ; After that, the activities of the three kind of protective enzymes declined signlficandy. In the treatment of 12 d, SOD were decreased to 1206.5, 1144.7, 1108.6 U/g, POD were decreased to 3145, 2876, 2753 U/（ g · min）, CAT were decreased to 587.2, 698.1, 584. 1 KU. The comprehensive evaluation showed that the drought resistant abilities of these three Cichoriwn intybus L. ordered as PA-82 〉 Puna Chicory 〉 PA-43.
出处
《西南农业学报》
CSCD
北大核心
2014年第3期1041-1044,共4页
Southwest China Journal of Agricultural Sciences
基金
贵州省科学技术基金项目(黔科合J字[2011]2165)
贵州省农业攻关项目(黔科合NY字[2009]3083
黔科合NY字[2012]3049)
贵州省育种专项(黔农育专字[2011]036)
关键词
菊苣
航天诱变
水分胁迫
保护酶
Cichorium imtybus
Space mutation
Water stress
Protective enzyme
