摘要
采用富集培养的方法,从广西大学家禽养殖场废弃羽毛处土样中筛选获得一株高效降解羽毛角蛋白的菌株GZD-23。经形态学、生理生化特性和16S rDNA序列分析,鉴定该菌株为枯草芽孢杆菌(Bacillus subtilis)。环境因素影响和酶学特性实验结果表明,蔗糖与蛋白胨是菌株GZD-23降解羽毛的最佳外加碳氮源,pH 8.0、温度30℃、转速100 r/min和羽毛含量20 g/L为其降解羽毛的最适反应条件。菌株GZD-23合成的角蛋白酶最适反应温度为60℃,最适pH为7.5;金属离子Cu2+、Hg2+、Fe2+、Co2+、Zn2+、Mn2+以及SDS、EDTA和高浓度TritonX-100、异丙醇和β-巯基乙醇对其酶活有不同程度抑制作用;低浓度TritonX-100、异丙醇和β-巯基乙醇对其酶活具有一定促进作用。
Using the method of enrichment culture, a strain GZD-23 was isolated from waste feather in soil samples of Guangxi University poultry farm which was able to efficiently degrade feather keratinase. It was identified as Bacillus subtilis, based on morphology, physiological and biochemical characteristics, and 16S rDNA sequence analysis. Experimental results of environmental factors and enzymology characteristics showed that the best carbon and nitrogen source of GZD-23 degrading feather were sucrose and peptone, the optimum reaction conditions of degrading feather was pH 8.0, temperature 30℃, rotate speed 100 r/min and feather content 20 g/L while the optimum reaction conditions of degrading feather keratinase was pH 7.5 and temperature 60℃. In varying degrees, it was inhibited by Cu2＋, Hg2＋, Fe2＋, Co2＋, Zn2＋, Mn2＋, SDS, EDTA, high concentration TritonX-100, isopropanol and β-mercaptoethanol, but activated by low concentration TritonX-100, isopropanol and β-mercaptoethanol.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2014年第7期49-54,共6页
Environmental Science & Technology
