摘要
利用同源重组基因敲除方法构建了枯草芽孢杆菌(Bacillus subtilis)S44 ituD基因同源突变株。菌落PCR、RT-PCR、SDS-PAGE结果均显示ituD基因缺失突变株构建成功。野生株与突变株的菌落形态、生长速率以及抑菌活性均有显著差异。抑菌活性试验结果显示,突变株抑菌活性显著减弱,成功构建的ituD基因突变株为进一步研究ituD基因的生物学功能奠定了基础。
To approach the relationship between the ituD gene and the antifungal activity of Bacillus subtilis S44,an isogenic knockout mutant of ituD gene was generated based on the principle of homologous recombination and confirmed by PCR,reverse transcription polymerase chain reaction and SDS-PAGE.The resulting mutant strain exhibited growth kinetics different from those of the WT parent strain upon cultivation in standard laboratory used in our in vitro assays.The morphology,growth rate and antifungal activity of wild type and mutant colony were significantly different.Antifungal activity results showed antifungal activity mutants were significandy reduced.The ituD mutant was successfully constructed for further study on ituD gene biological function basis.
出处
《湖北农业科学》
北大核心
2014年第6期1436-1439,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(30800733)
石河子大学自然科学创新团队项目(2011ZRKXTD-00204)
