摘要
目的 :分离日本血吸虫感染及免疫血清识别的成虫抗原 (AWA)中的特异蛋白带 ,为血吸虫病免疫诊断提供新的抗原分子。方法 :免疫印迹法分析AWA的特异蛋白带 ,电泳层析法分离靶抗原。结果 :获得了感染血清和免疫血清识别的 6 7kD蛋白。结论
Objective To isolate the specific protein bands which react to sera from rabbits infected with Schistosoma japonicum(Sj)and those immunized with Sj adult worm antigens (AWA) for immunodiagnosis of schistosomiasis. Methods AWA was analysed using SDS PAGE and immunoblotting, and 67kD protein was fractionated by electrophoretic chromatography. Results 67kD protein which reacted to rabbit sera infected with Sj and immunized with AWA was obtained after the fractionation of AWA through electrophoretic chromatography. Conclusions Electrophoretic chromatography is a useful tool for the fractionation of schistosome antigens.
出处
《湖南医科大学学报》
CSCD
北大核心
2002年第2期102-104,共3页
Bulletin of Hunan Medical University
基金
湖南省科技厅资助项目(0 0jzy2 115 )
关键词
日本血吸虫
成虫抗原
免疫印迹法
电泳
67kD蛋白
AWA
Schistosoma japonicum
adult worm antigens *
immunoblotting
electrophoresis
67kD protein *
