摘要
组织工程心脏瓣膜的构建需要良好的支架结构作为细胞生长和附着的载体 ,本研究探讨了去细胞后猪主动脉瓣瓣叶的生物力学性能和组织相容性 ,发现该去细胞方法可以完全去除瓣叶内的细胞成分 ,且去细胞后瓣叶的组织含水量增加 ,可溶性蛋白含量下降 ,而瓣叶的热皱缩温度、厚度、断裂伸长率和断裂强度均未发生明显变化。幼兔皮下埋植试验证明 ,宿主细胞可以沿纤维框架的纹理长入到瓣膜内。说明该去细胞瓣叶具有足够的力学强度和组织相容性 ,可用于构建组织工程心脏瓣叶。
Tissue engineering heart valves need a scaffold with good quality for cell growth and adherence. The psesent work was to investigate the biomechanics and biocompatibility of acellular porcine heart valves. The method used in this study could completely remove the cellular components in the porcine heart valve leaflets. Compared with fresh leaflets,the water content of acellular leaflets increased, and the soluble protein content decreased significantly. But the shrinkage temperature, thickness, stretch rate and strength at rupture of the acellular leaflets were not altered significantly compared with the fresh leaflets. After implanting the acellular leaflets subcutaneously in rabbits,it was proved that host cells could grow into the leaflets along the collagen fibers. These results suggested that acellular porcine heart valve leaflets could be used as a scaffold to construct tissue engineering heart valves with satisfacfory mechanical property and tissue compatibility.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第4期310-311,W001,共3页
Medical Journal of Chinese People's Liberation Army
