摘要
应用共聚焦显微镜和流式细胞仪双荧光标记法 ,体外研究不同转移性肺癌细胞与血管内皮细胞的粘附性 ,并检测药物对上述过程的影响。结果显示 ,高转移性肺癌细胞 (PG)与血管内皮细胞的粘附能力、CD4 4和CD5 4表达水平明显高于低转移性的肺癌细胞(D9) ;当血管内皮细胞被脂多糖激活后 ,PG细胞与其的粘附性较激活前明显增加 ;茶多酚对PG细胞与血管内皮细胞的粘附性具有明显的抑制作用。提示粘附分子在介导肺癌细胞与内皮细胞粘附及血行转移中起着十分重要的作用 。
Confocal microscope and flow cytometry were used to study the adhesion and the expression of adhesive molecules between different metastatic lung cancer cells and endothelial cells (EC) in vitro. Tea polyphenols (TP) were tested for their effect on tumor cell endothelial adhesion.The results showed that the adhesion and expression of CD44?CD54 were significantly higher in PG cells than those in D9 cells. Treatment of EC monolayers with LPS(0 5ug/ml) for six hours caused an increase in adhesion of PG to EC and adhesive molecules expression. TP could inhibit the adhesion of PG to EC and expressions of CD44 and CD54 in dose dependent manner. It suggested that adhesion molecules might play an important role in mediating the adhesion of lung carcinoma to EC and hematogenous metastasis; TP had an inhibitory effect on metastasis of lung cancer.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第4期339-340,共2页
Medical Journal of Chinese People's Liberation Army
